中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2008年
5期
276-279
,共4页
路艳艳%宣志强%李雪芝%杨锦蓉%王菁
路豔豔%宣誌彊%李雪芝%楊錦蓉%王菁
로염염%선지강%리설지%양금용%왕정
二甲基甲酰胺%肝细胞凋亡%Bcl-2%Bax%Caspase-3
二甲基甲酰胺%肝細胞凋亡%Bcl-2%Bax%Caspase-3
이갑기갑선알%간세포조망%Bcl-2%Bax%Caspase-3
Dimethylfonnamide%Hepatocyte Apoptosis%Bcl-2%Bax%Caspase-3
目的 初步探讨二甲基甲酰胺(DMF)对正常成人肝细胞的致凋亡作用和对Bcl-2、Bax、Caspase-3表达的影响.方法 以终浓度为0、50、100、150、200mmol/L的DMF作用于正常成人肝细胞12 h后,应用流式细胞术和western blotting法检测细胞凋亡率及Bcl-2、Bax和Caspase-3表达的变化.结果 与阴性对照组比较,各DMF剂量组凋亡率明显上升,差异有统计学意义(P＜0.01).随着染毒剂量的增加,肝细胞凋亡率上升,呈现剂量-反应关系.且染毒后随着DMF剂量的增加,Bcl-2表达水平下降,差异有统计学意义(P＜0.01).与对照组比较,Bax 表达水平无明显变化,Bcl-2/Bax比值随着DMF剂量增加而下降,200mmol/L剂量组与阴性对照组比较,差异有统计学意义(P＜0.01).在DMF150、200mmol/L剂量组可见到Caspase-3切割活化.结论 DMF可诱导肝细胞发生凋亡,其机制可能与Bcl-2/Bax比值表达下调、Caspase-3发生切割活化有关.
目的 初步探討二甲基甲酰胺(DMF)對正常成人肝細胞的緻凋亡作用和對Bcl-2、Bax、Caspase-3錶達的影響.方法 以終濃度為0、50、100、150、200mmol/L的DMF作用于正常成人肝細胞12 h後,應用流式細胞術和western blotting法檢測細胞凋亡率及Bcl-2、Bax和Caspase-3錶達的變化.結果 與陰性對照組比較,各DMF劑量組凋亡率明顯上升,差異有統計學意義(P＜0.01).隨著染毒劑量的增加,肝細胞凋亡率上升,呈現劑量-反應關繫.且染毒後隨著DMF劑量的增加,Bcl-2錶達水平下降,差異有統計學意義(P＜0.01).與對照組比較,Bax 錶達水平無明顯變化,Bcl-2/Bax比值隨著DMF劑量增加而下降,200mmol/L劑量組與陰性對照組比較,差異有統計學意義(P＜0.01).在DMF150、200mmol/L劑量組可見到Caspase-3切割活化.結論 DMF可誘導肝細胞髮生凋亡,其機製可能與Bcl-2/Bax比值錶達下調、Caspase-3髮生切割活化有關.
목적 초보탐토이갑기갑선알(DMF)대정상성인간세포적치조망작용화대Bcl-2、Bax、Caspase-3표체적영향.방법 이종농도위0、50、100、150、200mmol/L적DMF작용우정상성인간세포12 h후,응용류식세포술화western blotting법검측세포조망솔급Bcl-2、Bax화Caspase-3표체적변화.결과 여음성대조조비교,각DMF제량조조망솔명현상승,차이유통계학의의(P＜0.01).수착염독제량적증가,간세포조망솔상승,정현제량-반응관계.차염독후수착DMF제량적증가,Bcl-2표체수평하강,차이유통계학의의(P＜0.01).여대조조비교,Bax 표체수평무명현변화,Bcl-2/Bax비치수착DMF제량증가이하강,200mmol/L제량조여음성대조조비교,차이유통계학의의(P＜0.01).재DMF150、200mmol/L제량조가견도Caspase-3절할활화.결론 DMF가유도간세포발생조망,기궤제가능여Bcl-2/Bax비치표체하조、Caspase-3발생절할활화유관.
Objective To investigate the apoptosis-inducing effect of DMF on the human liver cells (HL-7702) in vitro.Methods Liver cells were exposed to different concentrations of DMF (0,50,100,150,200 mmol/L)for 12 hours.Apoptotic rate,the expression of Bax,Bcl-2 and Caspase-3 in liver cells were measured by FCM and western blotting respectively.Resuits The increase in apoptotic rate of hepatocytes in concentration-manner was shown after DMF treatment for 12 h.After treatment the expression of Bcl-2 was decreased steadily and lower than the control group(P＜0.01),the expression of Bax showed no significant difference among the groups of different dosage by one-factor analysis of variance(P＞0.05),as the increase of the dosage of DMF.The ratio of Bcl-2/Bax dropped with the dosage of DMF increasing,and the ratio in 200 mmol/L of DMF was significantly lower than that of the control(P＜0.01).The new lands of procaspase-3 in 150,200 mmol/L were observed,which demonstrated that there was active caspase-3.Conclusion DMF can induce apoptosis of cultured adult normal hepatocytes in vitro,and the mechanism might be related to the decrease of Bcl-2/Bax and the cleavage of Caspase-3.
