中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2010年
5期
550-553
,共4页
潘麓羽%曹建平%纪蓉%封阳%周媛媛%彭晓梅%刘杨%陈遐林%崔丹%朱巍%樊赛军
潘麓羽%曹建平%紀蓉%封暘%週媛媛%彭曉梅%劉楊%陳遐林%崔丹%硃巍%樊賽軍
반록우%조건평%기용%봉양%주원원%팽효매%류양%진하림%최단%주외%번새군
蒿甲醚%鼻咽癌%放射敏感性
蒿甲醚%鼻嚥癌%放射敏感性
호갑미%비인암%방사민감성
Artemether%Nasopharyngeal neoplasms%Radiosensitivity
目的 研究蒿甲醚(artemether)对人鼻咽癌CNE-1细胞放射敏感性的影响及其作用机理.方法 用MTT法检测蒿甲醚对CNE-1细胞生长的抑制效应;克隆形成法检测蒿甲醚对CNE-1细胞放射敏感性的影响;流式细胞仪检测不同处理因素对CNE-1细胞的细胞周期的影响;Western blot分析蒿甲醚对电离辐射诱导的CNE-1细胞中的细胞周期调控蛋白Cyclin B1和Weel表达水平的影响.结果 蒿甲醚可抑制CNE-1细胞的增殖,且药物浓度和作用时间相关;20 μmol/L蒿甲醚对CNE-1细胞有放射增敏作用,其放射增敏作用随作用时间的延长而延长,作用24 h后增敏作用最强,放射增敏比(SER)为1.481.流式细胞仪分析表明,单纯60Coγ射线照射后G2/M期细胞比例上升,而G0/G1期细胞比例下降;药物和射线联合作用后,与单纯照射组相比,G0/G1期细胞比例上升(t=4.59,P＜0.05),G2/M期细胞比例下降(t=10.60,P＜0.05).蒿甲醚可降低照射后CNE-1细胞内Weel蛋白表达水平,提高Cyclin B1蛋白表达水平.结论 低浓度蒿甲醚对CNE-1细胞有放射增敏作用;蒿甲醚通过改变细胞周期调控蛋白Cyclin B1和Weel表达水平,诱导G2/M期阻滞而发挥放射增敏作用.
目的 研究蒿甲醚(artemether)對人鼻嚥癌CNE-1細胞放射敏感性的影響及其作用機理.方法 用MTT法檢測蒿甲醚對CNE-1細胞生長的抑製效應;剋隆形成法檢測蒿甲醚對CNE-1細胞放射敏感性的影響;流式細胞儀檢測不同處理因素對CNE-1細胞的細胞週期的影響;Western blot分析蒿甲醚對電離輻射誘導的CNE-1細胞中的細胞週期調控蛋白Cyclin B1和Weel錶達水平的影響.結果 蒿甲醚可抑製CNE-1細胞的增殖,且藥物濃度和作用時間相關;20 μmol/L蒿甲醚對CNE-1細胞有放射增敏作用,其放射增敏作用隨作用時間的延長而延長,作用24 h後增敏作用最彊,放射增敏比(SER)為1.481.流式細胞儀分析錶明,單純60Coγ射線照射後G2/M期細胞比例上升,而G0/G1期細胞比例下降;藥物和射線聯閤作用後,與單純照射組相比,G0/G1期細胞比例上升(t=4.59,P＜0.05),G2/M期細胞比例下降(t=10.60,P＜0.05).蒿甲醚可降低照射後CNE-1細胞內Weel蛋白錶達水平,提高Cyclin B1蛋白錶達水平.結論 低濃度蒿甲醚對CNE-1細胞有放射增敏作用;蒿甲醚通過改變細胞週期調控蛋白Cyclin B1和Weel錶達水平,誘導G2/M期阻滯而髮揮放射增敏作用.
목적 연구호갑미(artemether)대인비인암CNE-1세포방사민감성적영향급기작용궤리.방법 용MTT법검측호갑미대CNE-1세포생장적억제효응;극륭형성법검측호갑미대CNE-1세포방사민감성적영향;류식세포의검측불동처리인소대CNE-1세포적세포주기적영향;Western blot분석호갑미대전리복사유도적CNE-1세포중적세포주기조공단백Cyclin B1화Weel표체수평적영향.결과 호갑미가억제CNE-1세포적증식,차약물농도화작용시간상관;20 μmol/L호갑미대CNE-1세포유방사증민작용,기방사증민작용수작용시간적연장이연장,작용24 h후증민작용최강,방사증민비(SER)위1.481.류식세포의분석표명,단순60Coγ사선조사후G2/M기세포비례상승,이G0/G1기세포비례하강;약물화사선연합작용후,여단순조사조상비,G0/G1기세포비례상승(t=4.59,P＜0.05),G2/M기세포비례하강(t=10.60,P＜0.05).호갑미가강저조사후CNE-1세포내Weel단백표체수평,제고Cyclin B1단백표체수평.결론 저농도호갑미대CNE-1세포유방사증민작용;호갑미통과개변세포주기조공단백Cyclin B1화Weel표체수평,유도G2/M기조체이발휘방사증민작용.
Objective To evaluate the effect of artemether on the cell cycle and the radiosensitivity in human nasopharyngeal carcinoma cell line CNE-1.Methods Cell growth inhibition was assessed with MTT.The method of colony-forming was used to detect the radiation sensitivity.Cell cycle distribution was analyzed by using flow cytometry.The protein expressions of clyclin B1 and Weei were detected by using Western blot.Results The growth of CNE-1 cells was inhibited in a dose-dependent manner.The concentration of 20 μmol/L artemether had radiosensitive effect on CNE-1 cells at 24 h after administration,and SER was 1.481.When CNE-1 cell was irradiated,the G2/M cells increased (t =4.59,P ＜ 0.05).After exposure to combination of artemether and irradiation,the G2/M cells were decreased (t= 10.60,P ＜ 0.05).Western blot showed that artemether increased the level of cyclin B1 expression and inhibited the level of Weel expression.Conclusions The noncytotoxic concentration of artemether could enhance radiosensitization of CNE-1 cells.The radiosensitivity enhancement of artemether might depend on the exposure time.The effect is most obvious when radiation is delivered 24 h after expose to artemetherr.The radiosensitizing effect could be related to apoptosis.
