中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2011年
4期
433-436
,共4页
宗兆文%任永川%沈岳%陈永华%冉新泽%史春梦%程天民
宗兆文%任永川%瀋嶽%陳永華%冉新澤%史春夢%程天民
종조문%임영천%침악%진영화%염신택%사춘몽%정천민
放射损伤%真皮多功能干细胞%血小板源性生长因子-AA
放射損傷%真皮多功能榦細胞%血小闆源性生長因子-AA
방사손상%진피다공능간세포%혈소판원성생장인자-AA
Radiation injury%Dermal multipotent stem cell%Platelet-derived growth factor-AA
目的 观察血小板源性生长因子-AA(PDGF-AA)处理能否增加真皮多能干细胞(dMSCs)向全身照射(TBI)大鼠骨髓的分布.方法 分离雄性大鼠dMSCs,向第3代dMSCs中加入10μg/L PDGF-AA,继续培养2 h后,Western blot检测dMSCs中tenascin-C的表达,Transwell小室观察dMSCs的迁移能力,并收集细胞静脉移植到雌性全身照射大鼠体内,伤后2周采用针对Y染色体的实时定量PCR法检测骨髓中dMSCs含量.以未处理的dMSCs作为对照.结果 与未处理的dMSCs相比,PDGF-AA处理可上调dMSCs中tenascin-C的表达,在骨髓提取液的趋化下迁移到Transwell小室下层的细胞多,移植后分布到骨髓的dMSCs数量为(1.79±0.13)×105个,明显高于未处理的(1.24±0.09)×105个(t=8.833,P＜0.01).结论 移植前用PDGF-AA处理dMSCs可增强其迁移能力,并可增加其向全身照射大鼠骨髓的分布.
目的 觀察血小闆源性生長因子-AA(PDGF-AA)處理能否增加真皮多能榦細胞(dMSCs)嚮全身照射(TBI)大鼠骨髓的分佈.方法 分離雄性大鼠dMSCs,嚮第3代dMSCs中加入10μg/L PDGF-AA,繼續培養2 h後,Western blot檢測dMSCs中tenascin-C的錶達,Transwell小室觀察dMSCs的遷移能力,併收集細胞靜脈移植到雌性全身照射大鼠體內,傷後2週採用針對Y染色體的實時定量PCR法檢測骨髓中dMSCs含量.以未處理的dMSCs作為對照.結果 與未處理的dMSCs相比,PDGF-AA處理可上調dMSCs中tenascin-C的錶達,在骨髓提取液的趨化下遷移到Transwell小室下層的細胞多,移植後分佈到骨髓的dMSCs數量為(1.79±0.13)×105箇,明顯高于未處理的(1.24±0.09)×105箇(t=8.833,P＜0.01).結論 移植前用PDGF-AA處理dMSCs可增彊其遷移能力,併可增加其嚮全身照射大鼠骨髓的分佈.
목적 관찰혈소판원성생장인자-AA(PDGF-AA)처리능부증가진피다능간세포(dMSCs)향전신조사(TBI)대서골수적분포.방법 분리웅성대서dMSCs,향제3대dMSCs중가입10μg/L PDGF-AA,계속배양2 h후,Western blot검측dMSCs중tenascin-C적표체,Transwell소실관찰dMSCs적천이능력,병수집세포정맥이식도자성전신조사대서체내,상후2주채용침대Y염색체적실시정량PCR법검측골수중dMSCs함량.이미처리적dMSCs작위대조.결과 여미처리적dMSCs상비,PDGF-AA처리가상조dMSCs중tenascin-C적표체,재골수제취액적추화하천이도Transwell소실하층적세포다,이식후분포도골수적dMSCs수량위(1.79±0.13)×105개,명현고우미처리적(1.24±0.09)×105개(t=8.833,P＜0.01).결론 이식전용PDGF-AA처리dMSCs가증강기천이능력,병가증가기향전신조사대서골수적분포.
Objective To observe whether dermal multipotent stem cells (dMSCs) treated with platelet-derived growth factor-AA ( PDGF-AA )could distribute more frequently to the bone marrow in rats of total body irradiation (TBI).Methods Male dMSCs were isolated and 10 μg/L PDGF-AA was added to the culture medium and further cultured for 2 h.Then the expression of tenascin-C were examined by Western blot, and the migration ability of dMSCs was assessed in transwell chamber.The pre-treated dMSCs were transplanted by tail vein injection into female rats administered with total body irradiation, and 2 weeks after transplantation, real-time PCR was employed to measure the amount of dMSCs in bone marrow.Non-treated dMSCs served as control.Results PDGF-AA treatment increased the expression of tenascin-C in dMSCs, made (1.79 ± 0.13) × 105 cells migrate to the lower chamber under the effect of bone marrow extract, and distributed to bone marrow in TBI rats, significantly more than ( 1.24 ± 0.09) ×105 in non-treated dMSCs (t = 8.833, P ＜ 0.0l ).Conclusions PDGF-AA treatment could enhance the migration ability of dMSCs and increase the amount of dMSCs in bone marrow of TBI rats after transplantation.
