中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2010年
5期
524-526
,共3页
屈维龙%唐来坤%田峰%汪祖林%宋立%俞仲伟%叶青%王珂%吴凤金%骆德兴
屈維龍%唐來坤%田峰%汪祖林%宋立%俞仲偉%葉青%王珂%吳鳳金%駱德興
굴유룡%당래곤%전봉%왕조림%송립%유중위%협청%왕가%오봉금%락덕흥
膀胱癌%去整合素金属蛋白酶-12%增殖细胞核抗原%免疫组织化学
膀胱癌%去整閤素金屬蛋白酶-12%增殖細胞覈抗原%免疫組織化學
방광암%거정합소금속단백매-12%증식세포핵항원%면역조직화학
Bladder carcinoma%Adamalysin-12%PCNA%Immunohistochemistry
目的 研究去整合素金属蛋白酶-12(ADAM-12)和增殖细胞核抗原(PCNA)在膀胱癌中的表达,并探讨其表达与膀胱癌发病之间的关系.方法 应用免疫组织化学的方法 检测43例膀胱癌、15例正常膀胱组织中ADAM-12及PCNA的表达情况,并分析其与膀胱癌患者临床特征及病理分期的关系.结果免疫染色标本中,ADAM-12在膀胱癌组织中的表达明显高于正常膀胱组织,两者比较差异有统计学意义(x2=11.241,P=0.010).病理分期高的膀胱癌组织表达明显高于分期低者(x2=22.368,P＜0.001).PCNA在膀胱癌中的表达明显高于正常膀胱组织,两者比较差异有统计学意义(x2=9.250,P=0.026).病理分期高的膀胱癌组织的表达明显高于分期低者(x2=10.665,P=0.014).ADAM-12与PCNA在膀胱癌中的表达呈正相关(r=0.997,P＜0.001).结论 膀胱癌组织中ADAM-12蛋白和PCNA呈过表达,可能与肿瘤的发生发展密切相关;ADAM-12是膀胱癌具有潜在价值的肿瘤标记物,可以成为一项新的临床观察指标.
目的 研究去整閤素金屬蛋白酶-12(ADAM-12)和增殖細胞覈抗原(PCNA)在膀胱癌中的錶達,併探討其錶達與膀胱癌髮病之間的關繫.方法 應用免疫組織化學的方法 檢測43例膀胱癌、15例正常膀胱組織中ADAM-12及PCNA的錶達情況,併分析其與膀胱癌患者臨床特徵及病理分期的關繫.結果免疫染色標本中,ADAM-12在膀胱癌組織中的錶達明顯高于正常膀胱組織,兩者比較差異有統計學意義(x2=11.241,P=0.010).病理分期高的膀胱癌組織錶達明顯高于分期低者(x2=22.368,P＜0.001).PCNA在膀胱癌中的錶達明顯高于正常膀胱組織,兩者比較差異有統計學意義(x2=9.250,P=0.026).病理分期高的膀胱癌組織的錶達明顯高于分期低者(x2=10.665,P=0.014).ADAM-12與PCNA在膀胱癌中的錶達呈正相關(r=0.997,P＜0.001).結論 膀胱癌組織中ADAM-12蛋白和PCNA呈過錶達,可能與腫瘤的髮生髮展密切相關;ADAM-12是膀胱癌具有潛在價值的腫瘤標記物,可以成為一項新的臨床觀察指標.
목적 연구거정합소금속단백매-12(ADAM-12)화증식세포핵항원(PCNA)재방광암중적표체,병탐토기표체여방광암발병지간적관계.방법 응용면역조직화학적방법 검측43례방광암、15례정상방광조직중ADAM-12급PCNA적표체정황,병분석기여방광암환자림상특정급병리분기적관계.결과면역염색표본중,ADAM-12재방광암조직중적표체명현고우정상방광조직,량자비교차이유통계학의의(x2=11.241,P=0.010).병리분기고적방광암조직표체명현고우분기저자(x2=22.368,P＜0.001).PCNA재방광암중적표체명현고우정상방광조직,량자비교차이유통계학의의(x2=9.250,P=0.026).병리분기고적방광암조직적표체명현고우분기저자(x2=10.665,P=0.014).ADAM-12여PCNA재방광암중적표체정정상관(r=0.997,P＜0.001).결론 방광암조직중ADAM-12단백화PCNA정과표체,가능여종류적발생발전밀절상관;ADAM-12시방광암구유잠재개치적종류표기물,가이성위일항신적림상관찰지표.
Objective To investigate the expression of adamalysin-12 (ADAM-12) and PCNA in human bladder carcinoma and to investigate their correlation with different grades and stages of bladder cancer.Methods Biopsies of 15 normal bladder and 43 bladder tumors were analyzed.Immunohistochemistry was conducted to detect the expression of ADAM12 and PCNA in the biopsies.Results Postive expression signals of ADAM12 were detected significantly higher in the bladder cancer biopsies than that in the normal ones (P = 0.010).Those with lower histological grade had a higher expression level of ADAM-12 compared to the higher histological grades (P ＜0.001 ).Positive expression signals of PCNA were detected significantly higher in the bladder cancer biopsies than that in the normal ones (P = 0.026).Those with lower histological grade had a higher expression level of PCNA (P =0.014).There was a positive correlation between the expression of ADAM-12 and PCNA in bladder cancer (r =0.997,P ＜ 0.001 ).Conclusions The overexpression of ADAM-12 and PCNA in the biopsies of bladder tumors shows that protein expression of ADAM-12 and PCNA correlated with tumor stage and grade.Furthermore,ADAM-12 may be a promising biomarker of bladder cancer in the clinical implication.
