中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2009年
3期
159-162
,共4页
海马%11β-羟基类固醇脱氢酶1%足三里%电针
海馬%11β-羥基類固醇脫氫酶1%足三裏%電針
해마%11β-간기류고순탈경매1%족삼리%전침
Hippocampus%11β-hydroxysteroid dehydrogenase type 1%Zusanli caupoint%Electroacupuncture
目的 观察应激大鼠海马11β-羟基类固醇脱氢酶1(11β-HSD1)的表达变化及电针足三里穴对海马11β-HSD1的调节作用,从而探讨电针调节大鼠下丘脑-垂体-肾上腺轴的可能机制.方法 将实验大鼠随机分为对照组、束缚组及电针组.对照组大鼠不给予任何特殊处理;束缚组大鼠将其束缚在一个特制圆筒型鼠笼内,双后肢固定于圆筒外使其完全不能活动;电针组大鼠则在上述束缚应激状态下,取其双侧足三里穴给予电针刺激.采用Western印迹杂交法观察3组大鼠海马11β-HSD1的变化情况.结果 束缚组大鼠海马内11β-HSD1表达水平明显高于对照组水平(P＜0.05);电针组海马内11β-HSD1表达水平较束缚组进一步增加,并且持续至针刺结束后3 h.结论 电针足三里穴能促进束缚应激大鼠海马11β-HSD1蛋白表达水平增加,可能与负反馈调节HPA轴功能有关.
目的 觀察應激大鼠海馬11β-羥基類固醇脫氫酶1(11β-HSD1)的錶達變化及電針足三裏穴對海馬11β-HSD1的調節作用,從而探討電針調節大鼠下丘腦-垂體-腎上腺軸的可能機製.方法 將實驗大鼠隨機分為對照組、束縳組及電針組.對照組大鼠不給予任何特殊處理;束縳組大鼠將其束縳在一箇特製圓筒型鼠籠內,雙後肢固定于圓筒外使其完全不能活動;電針組大鼠則在上述束縳應激狀態下,取其雙側足三裏穴給予電針刺激.採用Western印跡雜交法觀察3組大鼠海馬11β-HSD1的變化情況.結果 束縳組大鼠海馬內11β-HSD1錶達水平明顯高于對照組水平(P＜0.05);電針組海馬內11β-HSD1錶達水平較束縳組進一步增加,併且持續至針刺結束後3 h.結論 電針足三裏穴能促進束縳應激大鼠海馬11β-HSD1蛋白錶達水平增加,可能與負反饋調節HPA軸功能有關.
목적 관찰응격대서해마11β-간기류고순탈경매1(11β-HSD1)적표체변화급전침족삼리혈대해마11β-HSD1적조절작용,종이탐토전침조절대서하구뇌-수체-신상선축적가능궤제.방법 장실험대서수궤분위대조조、속박조급전침조.대조조대서불급여임하특수처리;속박조대서장기속박재일개특제원통형서롱내,쌍후지고정우원통외사기완전불능활동;전침조대서칙재상술속박응격상태하,취기쌍측족삼리혈급여전침자격.채용Western인적잡교법관찰3조대서해마11β-HSD1적변화정황.결과 속박조대서해마내11β-HSD1표체수평명현고우대조조수평(P＜0.05);전침조해마내11β-HSD1표체수평교속박조진일보증가,병차지속지침자결속후3 h.결론 전침족삼리혈능촉진속박응격대서해마11β-HSD1단백표체수평증가,가능여부반궤조절HPA축공능유관.
Objective To observe the expression of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) in the hippocampi of rats stressed by restraint,and adjustments to 11β-HSD1 expression in response to electroacupuncture.The mechanisms of adjustment of the hypothalamic-pituitary-adrenal axis(HPA) to electroacupuncture were also studied.Methods Rats were randomly divided into a control group,a group stressed by restraint and an electroacupuncture group.The rats in the control group received no treatment. The rats in the strssd group were put into a small columnar cage and their hind legs tied outside the cage. The electroacupuncture group, in addition to being restrained,received electroacupuncture at the Zusanli acupoints on both sides of their bodies. The rats were then sacrificed and their hippocampi were isolated and lysed. The expression of 11β-HSD1 in each hippocampus were observed using the Western blotting technique. Results The leves of expression of 11β-HSD1 in the hippocampi of the restrained group were significantly higher than those in the control group.After electroacupuncture,11β-HSD1 expression in the hippocampus increased further and lasted 3 h. Conclusion Electroacupuncture at the Zusanli acupoints (ST36) can increase 11β-HSD1 expression in the hippocampus of stressed rats, and this adjustment may be related to the HPA axis' negative feedback function.
