中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2011年
5期
328-331
,共4页
张治华%赵天兰%余道江%伍丽君%刘强%张为宝
張治華%趙天蘭%餘道江%伍麗君%劉彊%張為寶
장치화%조천란%여도강%오려군%류강%장위보
狭长窄蒂任意皮瓣%一氧化氮合酶%成活面积
狹長窄蒂任意皮瓣%一氧化氮閤酶%成活麵積
협장착체임의피판%일양화담합매%성활면적
Slender narrow pedicle flap%Nitric oxide synthase%Survival area
目的 通过在猪背部设计一定宽长比例的狭长窄蒂所携带不同大小的几组随意型皮瓣,在术后不同时间测定各组皮瓣内一氧化氮合酶(nitric oxide synthase,NOS)和CD34的表达量,并观察皮瓣的成活面积,揭示NOS、CD34表达量与皮瓣成活的关系,以探讨狭长窄蒂皮瓣的成活机制.方法 在5只白色实验猪的两侧背部均形成蒂部宽长比皆为2:4的狭长窄蒂皮瓣各5个,其面积分别为2 cm×2 cm、3 cm×3 cm、4 cm×4 cm、5 cm×5 cm、6 cm×6 cm.并依次命名为A、B、C、D、E皮瓣,其中A瓣为对照瓣.术后对皮瓣进行大体观察,并分别于术中即刻、术后3、5、7、14 d切取皮瓣远端外侧缘组织块,用于测定皮瓣内NOS、CD34表达量,术后14 d计算皮瓣存活面积.结果 A、B、C组皮瓣NOS、CD34的表达量随皮瓣面积的增大逐步升高,皮瓣完全存活(P＜0.05);D、E组皮瓣当皮瓣面积达到一定限度时,NOS、CD34表达量不再随皮瓣面积的增大而升高,表达相对恒定,皮瓣远端部分坏死(P＞0.05).结论 任意型皮瓣的成活与蒂的宽长比没有直接关系.一定宽长比例的狭长窄蒂所携带的随意皮瓣成活面积有其一定限度.
目的 通過在豬揹部設計一定寬長比例的狹長窄蒂所攜帶不同大小的幾組隨意型皮瓣,在術後不同時間測定各組皮瓣內一氧化氮閤酶(nitric oxide synthase,NOS)和CD34的錶達量,併觀察皮瓣的成活麵積,揭示NOS、CD34錶達量與皮瓣成活的關繫,以探討狹長窄蒂皮瓣的成活機製.方法 在5隻白色實驗豬的兩側揹部均形成蒂部寬長比皆為2:4的狹長窄蒂皮瓣各5箇,其麵積分彆為2 cm×2 cm、3 cm×3 cm、4 cm×4 cm、5 cm×5 cm、6 cm×6 cm.併依次命名為A、B、C、D、E皮瓣,其中A瓣為對照瓣.術後對皮瓣進行大體觀察,併分彆于術中即刻、術後3、5、7、14 d切取皮瓣遠耑外側緣組織塊,用于測定皮瓣內NOS、CD34錶達量,術後14 d計算皮瓣存活麵積.結果 A、B、C組皮瓣NOS、CD34的錶達量隨皮瓣麵積的增大逐步升高,皮瓣完全存活(P＜0.05);D、E組皮瓣噹皮瓣麵積達到一定限度時,NOS、CD34錶達量不再隨皮瓣麵積的增大而升高,錶達相對恆定,皮瓣遠耑部分壞死(P＞0.05).結論 任意型皮瓣的成活與蒂的寬長比沒有直接關繫.一定寬長比例的狹長窄蒂所攜帶的隨意皮瓣成活麵積有其一定限度.
목적 통과재저배부설계일정관장비례적협장착체소휴대불동대소적궤조수의형피판,재술후불동시간측정각조피판내일양화담합매(nitric oxide synthase,NOS)화CD34적표체량,병관찰피판적성활면적,게시NOS、CD34표체량여피판성활적관계,이탐토협장착체피판적성활궤제.방법 재5지백색실험저적량측배부균형성체부관장비개위2:4적협장착체피판각5개,기면적분별위2 cm×2 cm、3 cm×3 cm、4 cm×4 cm、5 cm×5 cm、6 cm×6 cm.병의차명명위A、B、C、D、E피판,기중A판위대조판.술후대피판진행대체관찰,병분별우술중즉각、술후3、5、7、14 d절취피판원단외측연조직괴,용우측정피판내NOS、CD34표체량,술후14 d계산피판존활면적.결과 A、B、C조피판NOS、CD34적표체량수피판면적적증대축보승고,피판완전존활(P＜0.05);D、E조피판당피판면적체도일정한도시,NOS、CD34표체량불재수피판면적적증대이승고,표체상대항정,피판원단부분배사(P＞0.05).결론 임의형피판적성활여체적관장비몰유직접관계.일정관장비례적협장착체소휴대적수의피판성활면적유기일정한도.
Objective To reveal the relationship between NOS,CD34 expression and the flap survival area,and to explore the survival mechanism of narrow strip flap,by designing different skin flaps with a slender narrow pedicle in a certain proportion of length to width on pigs' backs.Methods Five narrow flaps were formed on each side of the five white domestic pigs' back,of which pedicle width length ratio were 2 cm:4 cm,the area of the flaps was 2 cm × 2 cm,3 cm × 3 cm,4 cm ×4 cm,5 cm × 5 cm,and 6 cm × 6 cm,respectively,and named as flaps A,B,C,D,and E in turn.Besides,the flap A was taken as control.After the operation,the flaps were evaluated with general obveration,and NOS and CD34 expression and survival area were analyzed.Results For groups A,B and C flaps,the expression level of NOS and CD34 gradually elevated as the flap increased in size,and flaps completely survived (P＜0.05) ; For flaps in groups D and E,when distal end of flap was partial necrosis,the expression level of NOS and CD34 no longer elevated with the increase of skin flap area,and their expression was relatively constant (P＞0.05).Conclusions The survival area of the skin flaps is not in proportion to the width of its pedicle.Thus there is a maximum survival area of a skin flap carried by slender narrow pedicle in a certain proportion of length to width.
