中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2011年
4期
9-11
,共3页
陈占军%吉庆春%崔新征%宋静超%王振华%姜功前
陳佔軍%吉慶春%崔新徵%宋靜超%王振華%薑功前
진점군%길경춘%최신정%송정초%왕진화%강공전
LRIG1基因%EGFR基因%食管癌%逆转录聚合酶链反应
LRIG1基因%EGFR基因%食管癌%逆轉錄聚閤酶鏈反應
LRIG1기인%EGFR기인%식관암%역전록취합매련반응
LRIG1 gene%EGFR gene%Esophageal carcinoma%RT-PCR
目的 探讨富含亮氨酸重复序列免疫球蛋白样蛋白1(LRIG1)和表皮生长因子受体(EGFR)在食管鳞癌中的表达与意义.方法 应用逆转录聚合酶链反应(RT-PCR)方法检测46例食管癌肿瘤组织LRIG1和EGFR基因的表达情况,并与46例癌旁正常组织进行比较分析.结果 食管癌组织中LRIG1 mRNA的表达水平(0.35±0.04)低于相应癌旁正常组织(0.67±0.07),P﹤0.05;食管癌组织中EGFR mRNA的表达水平(2.23±0.25)高于相应癌旁正常组织(1.88±0.21),P﹤0.05;食管癌组织、相应癌旁正常组织中LRIG1 mRNA和EGFR mRNA的表达水平呈负相关,差异有统计学意义(r=-0.662,P﹤0.05).结论 LRIG1 mRNA在食管癌组织中存在低表达,提示其有可能具有抑癌作用.EGFR mRNA在食管癌组织中存在高表达,且LRIG1 mRNA和EGFR mRNA二者之间呈负相关,提示LRIG1 mRNA表达下降可能导致EGFR 活化,从而促进食管癌的发生发展.
目的 探討富含亮氨痠重複序列免疫毬蛋白樣蛋白1(LRIG1)和錶皮生長因子受體(EGFR)在食管鱗癌中的錶達與意義.方法 應用逆轉錄聚閤酶鏈反應(RT-PCR)方法檢測46例食管癌腫瘤組織LRIG1和EGFR基因的錶達情況,併與46例癌徬正常組織進行比較分析.結果 食管癌組織中LRIG1 mRNA的錶達水平(0.35±0.04)低于相應癌徬正常組織(0.67±0.07),P﹤0.05;食管癌組織中EGFR mRNA的錶達水平(2.23±0.25)高于相應癌徬正常組織(1.88±0.21),P﹤0.05;食管癌組織、相應癌徬正常組織中LRIG1 mRNA和EGFR mRNA的錶達水平呈負相關,差異有統計學意義(r=-0.662,P﹤0.05).結論 LRIG1 mRNA在食管癌組織中存在低錶達,提示其有可能具有抑癌作用.EGFR mRNA在食管癌組織中存在高錶達,且LRIG1 mRNA和EGFR mRNA二者之間呈負相關,提示LRIG1 mRNA錶達下降可能導緻EGFR 活化,從而促進食管癌的髮生髮展.
목적 탐토부함량안산중복서렬면역구단백양단백1(LRIG1)화표피생장인자수체(EGFR)재식관린암중적표체여의의.방법 응용역전록취합매련반응(RT-PCR)방법검측46례식관암종류조직LRIG1화EGFR기인적표체정황,병여46례암방정상조직진행비교분석.결과 식관암조직중LRIG1 mRNA적표체수평(0.35±0.04)저우상응암방정상조직(0.67±0.07),P﹤0.05;식관암조직중EGFR mRNA적표체수평(2.23±0.25)고우상응암방정상조직(1.88±0.21),P﹤0.05;식관암조직、상응암방정상조직중LRIG1 mRNA화EGFR mRNA적표체수평정부상관,차이유통계학의의(r=-0.662,P﹤0.05).결론 LRIG1 mRNA재식관암조직중존재저표체,제시기유가능구유억암작용.EGFR mRNA재식관암조직중존재고표체,차LRIG1 mRNA화EGFR mRNA이자지간정부상관,제시LRIG1 mRNA표체하강가능도치EGFR 활화,종이촉진식관암적발생발전.
Objective To investigate the expression of LRIG1 and epithelial growth factor receptor(EGFR)in esophageal carcinoma. Methods The expression of LRIG1 and EGFR were detected by RT-PCR in 46 cases of esophageal carcinoma and comparing with 46 cases normal para-cancer tissues. Results The expression level of LRIG1 mRNA in esophageal carcinoma tissues(0.35±0.04) was lower than that in in esophageal carcinoma tissues(2.23±0.25) was higher than that in normal expression level of LRIG1 mRNA and EGFR mRNA in esophageal carcinoma tissues and normal para-cancer tissues,there were significant differences between them(r=-0.662,P<0.05). Conclusions The expression level of LRIG1 mRNA is lower in esophageal carcinoma tissues than that in normal para-cancer tissues,which reveals that LRIG1 gene may be an anti-oncogene. The high expression level of EGFR mRNA in esophageal carcinoma tissues and negative correlation between the expression level of LRIG1 mRNA and EGFR mRNA in human esophageal tissues, may reveal that the lower expression of LRIG1 mRNA lead to the reduction of inhibition signal to EGFR and active it, results in the progression of esophageal carcinoma.
