中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2010年
11期
845-849
,共5页
孙风波%张佃良%郑红梅%宋波
孫風波%張佃良%鄭紅梅%宋波
손풍파%장전량%정홍매%송파
胃肿瘤%恶病质%白细胞介素10%多态性,单核苷酸
胃腫瘤%噁病質%白細胞介素10%多態性,單覈苷痠
위종류%악병질%백세포개소10%다태성,단핵감산
Stomach neoplasms%Cachexia%IL-10%Polymorphism,single nucleotide
目的 探讨白细胞介素10(IL-10)基因启动子区-1082、-819和-592位点的多态性及单体型与胃癌恶病质的关系.方法 将223例胃癌患者分为恶病质组107例,非恶病质组116例.用放射免疫学方法检测两组患者的血清IL-10水平,用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测两组患者IL-10基因-1082、-819和-592位点的单核苷酸多态性.结果 恶病质组血清IL-10水平为(91.64±13.25)ng/ml,较非恶病质组[(56.53±12.43)ng/ml]显著升高(P＜0.01).Logistic多因素回归分析结果显示,血清IL-10水平为胃癌恶病质发生的独立危险因素(OR=1.710,95%CI为1.285～2.276).恶病质组患者IL-10基因-1082AG和-819CC基因型频率较非恶病质组患者均显著升高(P=0.036,P=0.024).Logistic多因素回归分析结果显示,-1082AG基因型的胃癌患者发生恶病质的风险较-1082AA基因型者增加(OR=1.989,95%CI为1.041～3.802),-819CC基因型的胃癌患者发生恶病质的风险较-819TT基因型者增加(OR=3.393,95%CI为1.298～8.871).单体型分析显示,IL-10基因GCC单体型为胃癌患者发生恶病质的高危因素(OR=2.21,95%CI为1.14～4.30).结论 IL-10基因单体型与胃癌患者恶病质的发生有关.
目的 探討白細胞介素10(IL-10)基因啟動子區-1082、-819和-592位點的多態性及單體型與胃癌噁病質的關繫.方法 將223例胃癌患者分為噁病質組107例,非噁病質組116例.用放射免疫學方法檢測兩組患者的血清IL-10水平,用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)方法檢測兩組患者IL-10基因-1082、-819和-592位點的單覈苷痠多態性.結果 噁病質組血清IL-10水平為(91.64±13.25)ng/ml,較非噁病質組[(56.53±12.43)ng/ml]顯著升高(P＜0.01).Logistic多因素迴歸分析結果顯示,血清IL-10水平為胃癌噁病質髮生的獨立危險因素(OR=1.710,95%CI為1.285～2.276).噁病質組患者IL-10基因-1082AG和-819CC基因型頻率較非噁病質組患者均顯著升高(P=0.036,P=0.024).Logistic多因素迴歸分析結果顯示,-1082AG基因型的胃癌患者髮生噁病質的風險較-1082AA基因型者增加(OR=1.989,95%CI為1.041～3.802),-819CC基因型的胃癌患者髮生噁病質的風險較-819TT基因型者增加(OR=3.393,95%CI為1.298～8.871).單體型分析顯示,IL-10基因GCC單體型為胃癌患者髮生噁病質的高危因素(OR=2.21,95%CI為1.14～4.30).結論 IL-10基因單體型與胃癌患者噁病質的髮生有關.
목적 탐토백세포개소10(IL-10)기인계동자구-1082、-819화-592위점적다태성급단체형여위암악병질적관계.방법 장223례위암환자분위악병질조107례,비악병질조116례.용방사면역학방법검측량조환자적혈청IL-10수평,용취합매련반응-한제성편단장도다태성(PCR-RFLP)방법검측량조환자IL-10기인-1082、-819화-592위점적단핵감산다태성.결과 악병질조혈청IL-10수평위(91.64±13.25)ng/ml,교비악병질조[(56.53±12.43)ng/ml]현저승고(P＜0.01).Logistic다인소회귀분석결과현시,혈청IL-10수평위위암악병질발생적독립위험인소(OR=1.710,95%CI위1.285～2.276).악병질조환자IL-10기인-1082AG화-819CC기인형빈솔교비악병질조환자균현저승고(P=0.036,P=0.024).Logistic다인소회귀분석결과현시,-1082AG기인형적위암환자발생악병질적풍험교-1082AA기인형자증가(OR=1.989,95%CI위1.041～3.802),-819CC기인형적위암환자발생악병질적풍험교-819TT기인형자증가(OR=3.393,95%CI위1.298～8.871).단체형분석현시,IL-10기인GCC단체형위위암환자발생악병질적고위인소(OR=2.21,95%CI위1.14～4.30).결론 IL-10기인단체형여위암환자악병질적발생유관.
Objective To investigate whether the single nucleofide polymorphisms (SNPs) at -1082, -819 and -592 of interleukin-10 gene and its haplotype are associated with cachexia in patients with gastric cancer. Methods Radioimmunoassay was used to examine the serum levels of IL-10 in 223 patients with gastric cancer. The single nucleotide polymorphisms (SNPs) of IL-10 gene -1082G/A,-819T/C and -592A/C were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The serum levels of IL-10 were significantly higher in patients with cachexia than those without (P ＜0.001 ). An increased frequency of - 1082G allele was noted in patients with cachexia (P = 0. 049). The frequencies of -1082AG and -819CC genotypes were elevated in patients with cachexia than those without (P = 0.036, 0.024). In a logistic regression analysis adjusted for actual weight, carcinoma location and stage, the - 1082AG genotype was associated with an odds ratio of 1. 989 (95%CI, 1.041-3.802, P=0.037), and the -819CC genotype with an odds ratio of 3.393 (95% CI,1. 298-8.871, P= 0. 013 ) for cachexia. Furthermore, haplotype analysis revealed that G1082C819C592 haplotype was associated with a significantly increased risk of cachexia ( OR = 2.21; 95% CI, 1.14-4. 30;P =0.02). Conclusion Our results suggest that the gene haplotype of IL-10 contributes to the occurrence of cachexia in patients with gastric cancer in Chinese population.
