CAJ | 학술논문

目的调查天津地区5岁以下住院的腹泻患儿HAstV的感染情况及其分型特点.方法收集天津市儿童医院2008年5月1日至2009年4月30日住院腹泻患儿的粪便标本837份,用ELISA进行HAstV抗原检测,将检测阳性的标本进行细胞培养,产生细胞病变(CPE)后,提取病毒RNA,RT-PCR扩增并对产物进行测序确证.同时用胶体金免疫层析法进行轮状病毒抗原检测.结果 HAstV抗原阳性检出率为3.0%(25/837),与轮状病毒共同感染者的检出率为0.7%(6/837);96%(24/25)的HAstV腹泻发生在2岁以下患儿,其中小于6月龄的患儿占HAstV阳性标本的48%(12/25);HAstV感染主要集中在2008年8月至2009年4月,与轮状病毒流行季节相似;21例接种的HAstV抗原阳性的标本中,由RT-PCR成功检出11例,经测序确证均为HAstV-1型.结论 HAstV是天津地区2008-2009年婴幼儿病毒性腹泻的重要病原之一,流行型别以HAstV-1型为主.
목적 조사천진지구5세이하주원적복사환인HAstV적감염정황급기분형특점.방법 수집천진시인동의원2008년5월1일지2009년4월30일주원복사환인적분편표본837빈,용ELISA진행HAstV항원검측,장검측양성적표본진행세포배양,산생세포병변(CPE)후,제취병독RNA,RT-PCR확증병대산물진행측서학증.동시용효체금면역층석법진행륜상병독항원검측.결과 HAstV항원양성검출솔위3.0%(25/837),여륜상병독공동감염자적검출솔위0.7%(6/837);96%(24/25)적HAstV복사발생재2세이하환인,기중소우6월령적환인점HAstV양성표본적48%(12/25);HAstV감염주요집중재2008년8월지2009년4월,여륜상병독류행계절상사;21례접충적HAstV항원양성적표본중,유RT-PCR성공검출11례,경측서학증균위HAstV-1형.결론 HAstV시천진지구2008-2009년영유인병독성복사적중요병원지일,류행형별이HAstV-1형위주.
Objective To study the pathogenic prevalence and genotypes of astrovirus among children under 5 years old hospitalized with diarrhea in Tianjin. Methods A total 837 stool specimens were collected from children with diarrhea hospitalized in Tianjin children's hospital from May 2008 to April 2009. Astrovirus antigens were detected using ELISA and the postive specimens were inoculated in CaCo-2cells. After the CPE caused by virus were observed, the total RNA of virus was extracted, then the genomc fragments of the strains were amplified by using RT-PCR and confirmed by sequencing of the RT-PCR products. Detection of rotavirus was employed by Colloidal Gold Device. Results Astrovirus antigen was found positive in 3.0% of the patients. The coinfection rate of astrovirus and rotavirus was 0. 7% (6/837).Ninety-six persent of children with astrovirus diarrhea were younger than 2 years of age, Forty-eight persent of children with astrovirus diarrhea were younger than 6 months. The astrovirus infections occurred mainly between August 2008 and April 2009. Of the 21 astrovirus positive specimens, 11 cases were successfully identified by RT-PCR and they were all serotype 1. Conclusion Astrovirus is a major cause of nonbacterical diarrhea between 2008 and 2009 in Tianjin, and the predominant serotype is type 1.