CAJ | 학술논문

目的探讨含δ亚基的突触外GABAA受体在大鼠岛叶电刺激癫痫模型海马中的表达及意义.方法将48只成年雄性SD大鼠随机分为模型组、假手术组和正常对照组,模型组:通过慢性电刺激大鼠岛叶,建立点燃模型；假手术组:只植入电极,不行电刺激；正常对照组:不给予任何处理.各组按时间点断头取脑,应用尼氏染色检测癫痫大鼠与正常大鼠海马神经元的数目情况；应用免疫组织化学对δ亚基相关蛋白进行检测；应用实时荧光定量聚合酶链式反应对海马δ亚基mRNA进行检测.结果尼氏染色检测表明癫痫大鼠(99±8.7)与正常大鼠(103±7.9)海马齿状回神经元数目的差异无统计学意义(P＞0.05).实时荧光定量核酸扩增检测系统与免疫组织化学检测发现电点燃模型组在点燃成功后7d时海马δ亚基mRNA及蛋白均表现出明显的下降(P＜0.01).结论大鼠岛叶电刺激癫痫模型海马中δ亚基mRNA及相关蛋白表达显著减少,含δ亚基的突触外GABAA受体与岛叶癫痫的发生发展有着密切的联系.
목적 탐토함δ아기적돌촉외GABAA수체재대서도협전자격전간모형해마중적표체급의의.방법 장48지성년웅성SD대서수궤분위모형조、가수술조화정상대조조,모형조:통과만성전자격대서도협,건립점연모형；가수술조:지식입전겁,불행전자격；정상대조조:불급여임하처리.각조안시간점단두취뇌,응용니씨염색검측전간대서여정상대서해마신경원적수목정황；응용면역조직화학대δ아기상관단백진행검측；응용실시형광정량취합매련식반응대해마δ아기mRNA진행검측.결과 니씨염색검측표명전간대서(99±8.7)여정상대서(103±7.9)해마치상회신경원수목적차이무통계학의의(P＞0.05).실시형광정량핵산확증검측계통여면역조직화학검측발현전점연모형조재점연성공후7d시해마δ아기mRNA급단백균표현출명현적하강(P＜0.01).결론 대서도협전자격전간모형해마중δ아기mRNA급상관단백표체현저감소,함δ아기적돌촉외GABAA수체여도협전간적발생발전유착밀절적련계.
Objective To explore the expression of δ subunit-containing γ-aminobutyric acidA (GABA)A receptors in insular electrical kindled rats and analyze the significance of the findings.Methods A total of 48 male Sprague-Dawley rats were divided randomly into 3 groups:kindled,sham-operated and control. Kindled group:chronic insular electrical kindled models of rats established by electrical stimulation.Sham-operated group:the same method of the kindled group without electrical stimulation.Control group:without surgery.The number of hippocampal neurons of rat epilepsy,sham-operated and control groups were detected by Nissl staining.The hippocampal expressions of δ subunit of these groups were tested by immunohistochemistry.The mRNA of δ subunit of hippocampus of these groups was detected by quantitative-polymerase chain reaction (q-PCR).Results The findings of Nissl staining showed that there was no significant difference of the number of hippocampal neurons between epileptic and normal rats (P ＞0.05).The findings of q-PCR showed that mRNA of δ subunit of the hippocampus of sham-operated and control groups represented much more than that of kindled group at Day 7 post-kindling ( P ＜ 0.01 ).The findings of immunohistochemistry showed that the hippocampal expression of δ subunit of sham-operated and control groups represented much more than that of kindled group at Day 7 post-kindling ( P ＜ 0.01 ).Conclusion The hippocampal expression of δ subunit-containing GABAA receptors decreases in insular epilepsy.It may play an important role in the occurrence and development of insular epilepsy.