中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2001年
1期
108-110
,共3页
弧菌科%噬菌体%鉴定
弧菌科%噬菌體%鑒定
호균과%서균체%감정
目的 简化副溶血弧菌等6种弧菌科常见病原菌的检测程序，缩短报告时间，提高防治效果。方法 从外环境标本分离筛选6种特异性噬菌体来诊断其相应病原菌并进行分型。结果 从1400份外环境标本分离到440株噬菌体，从中筛选出54株分别组成诊断和分型噬菌体组，经大量实验室和现场的菌株试用，效果良好，对各相应病原菌的诊断和分型率达80%～90%。结论 应用特异性噬菌体诊断相应病原菌，方法简便、快速、廉价实用，可节省大量实验材料，提高工作效率；用于分型是流行病学调查中追索传染源和传播途经的有效手段。
目的 簡化副溶血弧菌等6種弧菌科常見病原菌的檢測程序，縮短報告時間，提高防治效果。方法 從外環境標本分離篩選6種特異性噬菌體來診斷其相應病原菌併進行分型。結果 從1400份外環境標本分離到440株噬菌體，從中篩選齣54株分彆組成診斷和分型噬菌體組，經大量實驗室和現場的菌株試用，效果良好，對各相應病原菌的診斷和分型率達80%～90%。結論 應用特異性噬菌體診斷相應病原菌，方法簡便、快速、廉價實用，可節省大量實驗材料，提高工作效率；用于分型是流行病學調查中追索傳染源和傳播途經的有效手段。
목적 간화부용혈호균등6충호균과상견병원균적검측정서，축단보고시간，제고방치효과。방법 종외배경표본분리사선6충특이성서균체래진단기상응병원균병진행분형。결과 종1400빈외배경표본분리도440주서균체，종중사선출54주분별조성진단화분형서균체조，경대량실험실화현장적균주시용，효과량호，대각상응병원균적진단화분형솔체80%～90%。결론 응용특이성서균체진단상응병원균，방법간편、쾌속、렴개실용，가절성대량실험재료，제고공작효솔；용우분형시류행병학조사중추색전염원화전파도경적유효수단。
Objective To predigest the detection procedure of 6 species ofpathogenic bacteria of Vibrionaceae. Methods Six species of specific bacteriophages were isolated and filtered to identify and type corresponding pathogenic bacteria. Results 440 strains of bacteriophages, among which 54 strains were selected to form the typing bacteriophage groups, were isolated from 1400 environmental specimens. The typing bacteriophage groups could identify and type 80%-90% of bacteria in laboratory or those from environment. Conclusion The application of specific bacteriophages to identify the corresponding pathogenic bacteria is fast, economic and easy to operate, so bacteriophage typing is an effective method in epidemiology investigation.
