CAJ | 학술논문

目的研究慢性阻塞性肺疾病(COPD)患者维生素D结合蛋白(VDBP)基因多态性,从而探讨COPD的发病机制.方法 COPD组75例和健康对照组69例,提取外周静脉血白细胞DNA,利用聚合酶链反应(PCR)和限制性内切酶技术鉴别VDBP基因型,比较两组间VDBP基因型的分布和表达.结果在COPD组,等位基因1F出现频率高于对照组(58%vs39%,P=0.001),其1F-1F纯合子明显高于对照组(32%vs11.6%,P=0.003).在COPD组,等位基因2出现率低于对照组(20%vs38.0%,P=0.001),其纯合子2-2明显低于对照组(2.7%vs18.8%,P=0.004).结论 COPD发病与VDBP基因表达和分布有关,1F等位基因可能是COPD发病的危险因素,1F-1F基因型可能是高危因素.2-2基因型可能是保护因素.
목적 연구만성조새성폐질병(COPD)환자유생소D결합단백(VDBP)기인다태성,종이탐토COPD적발병궤제.방법 COPD조75례화건강대조조69례,제취외주정맥혈백세포DNA,이용취합매련반응(PCR)화한제성내절매기술감별VDBP기인형,비교량조간VDBP기인형적분포화표체.결과 재COPD조,등위기인1F출현빈솔고우대조조(58%vs39%,P=0.001),기1F-1F순합자명현고우대조조(32%vs11.6%,P=0.003).재COPD조,등위기인2출현솔저우대조조(20%vs38.0%,P=0.001),기순합자2-2명현저우대조조(2.7%vs18.8%,P=0.004).결론 COPD발병여VDBP기인표체화분포유관,1F등위기인가능시COPD발병적위험인소,1F-1F기인형가능시고위인소.2-2기인형가능시보호인소.
Objective Detection of vitamin D binding protein(VDBP)genetical polymorphism in chronic obstructive pulmonary di8ease(COPD).Methods Blood asmples were taken from seventy-five with COPD patients and sixty-nine in controls.DNA was extracted form the white blood ceLls of both groups.Genotypes of VDBP were meagured with polymerage chain reaction(PCR)and restriction fragment length polymorphism analysis.Results The frequency of allele 1F in COPD group was higher than that in group control(58%vs39%,P =0.001),the proportion of allele 1F homozygote wag significantly higher in COPD group than that in group control(32%vs11.6%,P=0.003).In COPD group the frequency of allele 2 was significandy lower than that in group control(20%vs38.0%.P=0.001).Phenotype 2-2 showed a decreasing in group COPD.Conclusion The VDBP polymorphism was associated with COPD,allele 1F may increase the risk of COPD,1F homozygote is one of the most risk factors for COPD,The protective effect of COPD was 2-2 homozygote.