CAJ | 학술논문

目的通过冠状动脉内注射月桂酸钠,观察月桂酸钠对缺血性心肌冠状动脉微血管血栓形成和心肌形态学变化的影响.方法雄性SD大鼠54只,体质量(250±30)g.将大鼠按体质量随机分为3组:月桂酸钠组、假手术组、对照组,每组18只.将大鼠开胸,分离腹主动脉,在夹闭腹主动脉的同时,月桂酸钠组向其根部注入月桂酸钠(1 mg/kg),在20 s后立即松夹;假手术组注入等体积生理盐水,对照组不做任何处理.每组分别于术后第1、7、28天处死4～6只大鼠,取心肌组织制成切片,经HE与马休黄-酸性品红-苯胺蓝染色,光镜下观察心肌冠状动脉微血管血栓形成及受损心肌的形态学变化,透射电镜下观察血管内皮细胞超微结构的变化.结果月桂酸钠组大鼠心肌冠状动脉微血管中,可见多量由血小板纤维蛋和胶原构成的血栓;假手术组、对照组大鼠微血管中未见血栓形成.月桂酸钠组冠状动脉微血管内皮细胞超微结构较假手术组、对照组改变明显,细胞间紧密连接增宽,微血管挛缩狭窄.月桂酸钠组随时间延长心肌炎纤维化加重,假手术组、对照组未见明显变化.结论心肌冠状动脉微血管血栓形成是月桂酸钠致缺血性心肌损伤的主要原因,内皮损伤可能是月桂酸钠导致冠状动脉微血管血栓形成的主要机制.
목적 통과관상동맥내주사월계산납,관찰월계산납대결혈성심기관상동맥미혈관혈전형성화심기형태학변화적영향.방법 웅성SD대서54지,체질량(250±30)g.장대서안체질량수궤분위3조:월계산납조、가수술조、대조조,매조18지.장대서개흉,분리복주동맥,재협폐복주동맥적동시,월계산납조향기근부주입월계산납(1 mg/kg),재20 s후립즉송협;가수술조주입등체적생리염수,대조조불주임하처리.매조분별우술후제1、7、28천처사4～6지대서,취심기조직제성절편,경HE여마휴황-산성품홍-분알람염색,광경하관찰심기관상동맥미혈관혈전형성급수손심기적형태학변화,투사전경하관찰혈관내피세포초미결구적변화.결과 월계산납조대서심기관상동맥미혈관중,가견다량유혈소판섬유단화효원구성적혈전;가수술조、대조조대서미혈관중미견혈전형성.월계산납조관상동맥미혈관내피세포초미결구교가수술조、대조조개변명현,세포간긴밀련접증관,미혈관련축협착.월계산납조수시간연장심기염섬유화가중,가수술조、대조조미견명현변화.결론 심기관상동맥미혈관혈전형성시월계산납치결혈성심기손상적주요원인,내피손상가능시월계산납도치관상동맥미혈관혈전형성적주요궤제.
Objective To observe the morphological changes of myocardium and intracoronary microthrombus by intracoronary injection of sodium laurate. Methods Fifty four male SD rats, weighing (250 ± 30)g,were randomly divided into 3 groups according to weight: sodium laurate group, sham group and control group,eighteen rats in each group. In sodium laurate group, exposed and occluded the aorta while injected with sodium laurate(1 mg/kg) for 20 seconds. In sham group, took normal saline instead and no operation in control group. At the 1st, 7th and 28th day after the operation, 4 - 6 rats were selected randomly from each group and euthanized. The cardiac tissue were obtained and fixed for pathological study. After HE staining and modified Lendrum staining,micro-thrombosis and morphological changes of injured myocardium were detected under light microscope. The ultrastructure change of vascular endothelial cells was observed with transmission electron microscopy (TEM).Results Microthrombus consisting of platelet fibers and collagen could be observed only in sodium laurate group.The ultrastroctural changes of endothelial cells were significant in sodium laurate group compared with other two groups. TEM analysis showed that tight junctions between cells became wide and microvascular became narrowed in sodium laurate group. The fibrosis changes were enhanced in sodium laurate group compared with sham group and control group with the time prolong. Conclusions Micro-thrombosis is one of mechanisms in the development of sodium laurate induced ischemic myocardial injury. Endothelial injury may be the mechanism for sodium laurate leading to microthrombus.