CAJ | 학술논문

目的观察我院呼吸重症监护病房(RICU)临床分离多重耐药铜绿假单胞菌β-内酰胺酶基因和Ⅰ类整合子标志物(qacE△1-sul1)存在状况.采用多基因聚类分析方法进行多重耐药菌株亲缘性分析.方法收集RICU分离多重耐药铜绿假单胞菌33株,采用PCR方法检测8种β-内酰胺酶基因(TEM、SHV、PER、DHA、IMP、VIM、OXA-23、OXA-24)和qacE△1-sul1基因共9种.应用多基因聚类分析法分析菌株亲缘性.结果 33株多重耐药铜绿假单胞菌中blaTEM阳性8株(24.24%),blaVIM阳性6株(18.19%),blaIMP阳性3株(9.09%),blaSHV阳性2株(6.06%),blaDHA阳性5株(15.15%),blaOXA-23,blaOXA-24,blaPER均未检出,qacE△1-sul1基因阳性28株(84.85%).多基因聚类分析发现存在克隆传播现象.结论我院RICU多重耐药铜绿假单胞菌携带多种β-内酰胺酶基因,以携带blaTEM基因为主.qacE△1-sul1携带率高,聚类分析显示存在克隆传播现象,应引起临床重视.
목적 관찰아원호흡중증감호병방(RICU)림상분리다중내약동록가단포균β-내선알매기인화Ⅰ류정합자표지물(qacE△1-sul1)존재상황.채용다기인취류분석방법진행다중내약균주친연성분석.방법 수집RICU분리다중내약동록가단포균33주,채용PCR방법검측8충β-내선알매기인(TEM、SHV、PER、DHA、IMP、VIM、OXA-23、OXA-24)화qacE△1-sul1기인공9충.응용다기인취류분석법분석균주친연성.결과 33주다중내약동록가단포균중blaTEM양성8주(24.24%),blaVIM양성6주(18.19%),blaIMP양성3주(9.09%),blaSHV양성2주(6.06%),blaDHA양성5주(15.15%),blaOXA-23,blaOXA-24,blaPER균미검출,qacE△1-sul1기인양성28주(84.85%).다기인취류분석발현존재극륭전파현상.결론 아원RICU다중내약동록가단포균휴대다충β-내선알매기인,이휴대blaTEM기인위주.qacE△1-sul1휴대솔고,취류분석현시존재극륭전파현상,응인기림상중시.
Objective To investigate the positive rate of beta-lactamase and qac△1-sul1 genes by polymerase chain reaction (PCR) method among clinical isolated strains of multi-drug resistant Pseudomonas aeruginosa. The strains'consanguinity were investigated by multi-genes cluster analysis.Methods Thirty-three strains of multi-drug resistant Pseudomonas aeruginosa were isolated from hospitalized patients in RICU. β-Lactamase genes including TEM, SHV, PER, DHA, IMP, VIM, OXA-23,OXA-24 and qacE△ 1-sul1, were detected by PCR amplication. The strains' homology were investigated by multi-genes cluster analysis. Results PCR results showed the positive rates of genes blaTEM, blalMP,blaVIM, blaSHV, blaDHA, and qacE△1-sul1 in multi-drug resistant Pseudomonas aeruginosa were 24.24 %, 18.19% ,9.09%, 6.06%, 15.15% and 84.85% respectively, while genes blaOXA-23, blaOXA-24 and blaPER were all negative. Polygene cluster analysis results discovered that there is a clone transmitted phenomenon. Conclusions Multi-drug resistant Pseudomonas aeruginosa isolated in RICU have carried various beta-lactamases genes and Multi-drug resistant Pseudomonas aeruginosa mainly carried blaTEM. We must pay attention to the spread of clones occurred in our RICU and high positive percentages of qacE△1-sul1 in multi-drug resistant Pseudomonas aeruginosa.