中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2008年
10期
991-993
,共3页
倪秀芹%李星%冯佳%赵玲辉%纪长伟
倪秀芹%李星%馮佳%趙玲輝%紀長偉
예수근%리성%풍가%조령휘%기장위
心肌梗死%神经生长因子%肾上腺素能神经
心肌梗死%神經生長因子%腎上腺素能神經
심기경사%신경생장인자%신상선소능신경
Myocardial infarction%Nerve growth factor%Adrenergic nerve
目的 探讨神经生长因子对心肌梗死后大鼠肾上腺素能神经纤维的保护作用及其机制.方法 实验用Wister大鼠120只,分为假手术对照组、心肌梗死模型组、神经生长因子组.分别于术后6 h及2、4、7、14 d取材,以免疫组织化学方法 显示肾上腺素能神经纤维,应用多功能真彩色病理图像分析系统分析肾上腺素能神经纤维的密度.结果 ①心肌梗死组心肌梗死区及梗死周围区肾上腺素能神经纤维密度在术后6h及2、4、7、14d均低于假手术组.②神经生长因子组术后7、14d心肌梗死区及梗死周围区肾上腺素能神经纤维密度较心肌梗死组明显升高.结论 神经生长因子对大鼠急性心肌梗死后心肌肾上腺素能神经具有神经保护作用.
目的 探討神經生長因子對心肌梗死後大鼠腎上腺素能神經纖維的保護作用及其機製.方法 實驗用Wister大鼠120隻,分為假手術對照組、心肌梗死模型組、神經生長因子組.分彆于術後6 h及2、4、7、14 d取材,以免疫組織化學方法 顯示腎上腺素能神經纖維,應用多功能真綵色病理圖像分析繫統分析腎上腺素能神經纖維的密度.結果 ①心肌梗死組心肌梗死區及梗死週圍區腎上腺素能神經纖維密度在術後6h及2、4、7、14d均低于假手術組.②神經生長因子組術後7、14d心肌梗死區及梗死週圍區腎上腺素能神經纖維密度較心肌梗死組明顯升高.結論 神經生長因子對大鼠急性心肌梗死後心肌腎上腺素能神經具有神經保護作用.
목적 탐토신경생장인자대심기경사후대서신상선소능신경섬유적보호작용급기궤제.방법 실험용Wister대서120지,분위가수술대조조、심기경사모형조、신경생장인자조.분별우술후6 h급2、4、7、14 d취재,이면역조직화학방법 현시신상선소능신경섬유,응용다공능진채색병리도상분석계통분석신상선소능신경섬유적밀도.결과 ①심기경사조심기경사구급경사주위구신상선소능신경섬유밀도재술후6h급2、4、7、14d균저우가수술조.②신경생장인자조술후7、14d심기경사구급경사주위구신상선소능신경섬유밀도교심기경사조명현승고.결론 신경생장인자대대서급성심기경사후심기신상선소능신경구유신경보호작용.
Objective To confirmthe protective effect of nerve growth factor (NGF)on cadiac adrenergie nerve in rats after acute myocardial infarction(AMI)and its mechanism.Methods 120 Wister rats were divided in-to sham-operated group.AMI group and NGF group.The samples were taken after6 h, d,4 d-7 d and 14 d sepa-lately.Immunohistochemistry method WaS used to show the distribution of adrenergic nerve fibers.The density of them were calculated by Medical Pathobgical Image Analysis Processing System.Results ①The densities of adrenergic nerve fibers in AMI group were obviously lower than that of sham-operated group greatly in 6 h and 2 d.4 d-7 d and14 d later.②The densities of adrenergic innervation in NGF group were obviously higher than that of AMI group 7 dand 14 d later.Conclusion NGF has protection effect 011 cardLac adrenergic nerve after AMI in the rat.
