CAJ | 학술논문

[目的]测定一株鸡传染性法氏囊病病毒基因组序列及其分子特征。[方法]分离出一株具有特殊分子特征的鸡传染性法氏囊病病毒（IBDV）超强毒株（vvIBDV）HLJ-0504,并对其基因组进行了克隆和测序。[结果]序列分析显示,HLJ-0504的基因组A节段属于超强毒株,而其B节段则来源于另外一个独特的祖先。动物实验表明,HLJ-0504对SPF雏鸡的致病率和致死率分别为100%和86.7%。[结论]具有独特基因组B节段的vvIBDV仍在我国流行,我国IBDV的进化特征存在多样性。IBDV的毒力不是由基因组的A或B单独决定的。
[목적]측정일주계전염성법씨낭병병독기인조서렬급기분자특정。[방법]분리출일주구유특수분자특정적계전염성법씨낭병병독（IBDV）초강독주（vvIBDV）HLJ-0504,병대기기인조진행료극륭화측서。[결과]서렬분석현시,HLJ-0504적기인조A절단속우초강독주,이기B절단칙래원우령외일개독특적조선。동물실험표명,HLJ-0504대SPF추계적치병솔화치사솔분별위100%화86.7%。[결론]구유독특기인조B절단적vvIBDV잉재아국류행,아국IBDV적진화특정존재다양성。IBDV적독력불시유기인조적A혹B단독결정적。
[Objective] The paper was to determine the genomic sequence of a very virulent strain of infectious bursal disease virus（IBDV）,and study its molecular characteristics.[Method] A very virulent strain（vvIBDV）（HLJ-0504） of infectious bursal disease virus（IBDV） with special characters was isolated in China and its genome was sequenced.[Result] Sequence analysis showed that segment A of HLJ-0504 was derived from vvIBDV,while segment B was from a distinct ancestor.The morbidity and mortality of HLJ-0504 was 100% and 86.7%to SPF chickens,respectively.[Conclusion] vvIBDV with distinct segment B were still circulating and the evolution of IBDV was diversified in China.Besides,it is hard to imagine that the virulence of IBDV is determined solely by segment A or B.