河北化工
河北化工
하북화공
HEBEI CHEMICAL ENGINEERING AND INDUSTRY
2011年
12期
11-15
,共5页
杨立霞%朱欣杰%修建新%李锦
楊立霞%硃訢傑%脩建新%李錦
양립하%주흔걸%수건신%리금
2-酮基-L-古龙酸%L-山梨糖%醇醛脱氢酶%两步发酵法%生酮基古龙酸DSM4025
2-酮基-L-古龍痠%L-山梨糖%醇醛脫氫酶%兩步髮酵法%生酮基古龍痠DSM4025
2-동기-L-고룡산%L-산리당%순철탈경매%량보발효법%생동기고룡산DSM4025
2-keto-L-gulonic acid%L-sorbose%alcohol/aldehyde dehydrogenases%two-step fermentation%ketogulonicigenium vulgare DSM4025
生酮基古龙酸菌是两步发酵法生产维生素C（VC）过程中的关键菌株,其能够充分转化L-山梨糖生成2-KGA,KV菌的转化代谢机制和氧化还原酶系一直是VC生产中的研究热点。KV菌的全基因组序列已经测定完成,基因注释工作正在积极推进。综述了VC生产研究进展、两步发酵法及KV菌发子生物学研究进展。指出随着研究工作的深入,越来越多的在转化L-山梨糖生成2-KGA过程中起关键作用的醇醛脱氢酶被发现。KV菌分子生物学方面的进展为革新VC生产工艺,大幅度降低生产成本,为我国的VC产业在竞争中获胜奠定了基础。
生酮基古龍痠菌是兩步髮酵法生產維生素C（VC）過程中的關鍵菌株,其能夠充分轉化L-山梨糖生成2-KGA,KV菌的轉化代謝機製和氧化還原酶繫一直是VC生產中的研究熱點。KV菌的全基因組序列已經測定完成,基因註釋工作正在積極推進。綜述瞭VC生產研究進展、兩步髮酵法及KV菌髮子生物學研究進展。指齣隨著研究工作的深入,越來越多的在轉化L-山梨糖生成2-KGA過程中起關鍵作用的醇醛脫氫酶被髮現。KV菌分子生物學方麵的進展為革新VC生產工藝,大幅度降低生產成本,為我國的VC產業在競爭中穫勝奠定瞭基礎。
생동기고룡산균시량보발효법생산유생소C（VC）과정중적관건균주,기능구충분전화L-산리당생성2-KGA,KV균적전화대사궤제화양화환원매계일직시VC생산중적연구열점。KV균적전기인조서렬이경측정완성,기인주석공작정재적겁추진。종술료VC생산연구진전、량보발효법급KV균발자생물학연구진전。지출수착연구공작적심입,월래월다적재전화L-산리당생성2-KGA과정중기관건작용적순철탈경매피발현。KV균분자생물학방면적진전위혁신VC생산공예,대폭도강저생산성본,위아국적VC산업재경쟁중획성전정료기출。
Ketogulonigenium vulgare was the critical strain in the VC production by two-step fermentation method,which was responsible for the conversion reaction of L-Sorbose t o 2-KGA.In VC production,the research was always focused on the ketogulonigenium vulgare＇s metabolic pathway and oxidase/reductases.The complete genome sequence of ketogulonicigenium vulgare was determined.Gene annotation work has been promoting actively.The production and research progress of VC and KV＇s molecular biology,two-step fermentation method of VC were summaried.It is pointed out that with the deeper research,more and more alcohol/ aldehyde dehydrogenase were found that play a key role in the transformation process of L-sorbose to 2-KGA.KV＇s molecular biology progression innovates VC industry and greatly reduces the production cost,which will make our VC industry won in the competition.
