CAJ | 학술논문

[目的]对海栖热袍菌电转化技术进行研究。[方法]采用不同参数即不同的方型电脉冲参数、指数衰退型电脉冲参数和高电压电击参数对海栖热袍菌感受态细胞进行电转化,得到的转化子进行PCR验证。[结果]试验中发现以150V的低压方形电脉冲,室温厌氧条件下电击25ms、1次,有利于T.maritima MS8的转化,但这种以整合质粒进行的转化效率极低。[结论]为提高T.maritima MS8的基因转化效率奠定了基础。
[목적]대해서열포균전전화기술진행연구。[방법]채용불동삼수즉불동적방형전맥충삼수、지수쇠퇴형전맥충삼수화고전압전격삼수대해서열포균감수태세포진행전전화,득도적전화자진행PCR험증。[결과]시험중발현이150V적저압방형전맥충,실온염양조건하전격25ms、1차,유리우T.maritima MS8적전화,단저충이정합질립진행적전화효솔겁저。[결론]위제고T.maritima MS8적기인전화효솔전정료기출。
[Objective] The aim was to study on the electrotransformation of Thermotoga maritima MS8.[Method] Square waves,exponential waves and high voltage shock were used for the electrotransformation of T.maritima MS8,and the obtained transformants were detected by PCR.[Result] A single square electric pulse could be applied to cell sample in 0.2 cm ET cuvettes by using a Bio-Rad Gene Pulser set at 150 V,25 ms at room temperature,but the transformation efficiency was very low.[Conclusion] This research may improve the transformation efficiency of T.maritima MS8.