CAJ | 학술논문

为了解人参属植物EST中SSR分布特点及其在三七SSR标记中的应用,利用生物信息学方法,用primer3软件对dbEST数据库中人参属植物人参、西洋参和三七的EST序列进行搜索,发现人参属植物EST-SSR出现频率为11.54%,平均每4.39kb出现1个SSR。人参属植物单核苷酸重复基元占主导地位,其次为三核苷酸重复基元和二核苷酸重复基元,分别占总SSR的54.48%、17.31%和16.36%。人参属EST-SSR的优势类型为A/T和AT/TA,分别占54.73%和8.21%。根据人参属植物EST中的SSR设计48对引物,在合适的PCR扩增体系下,用5个三七样品的DNA为模板进行PCR扩增,引物有效扩增率85.42%,其中多态性引物占可扩增引物的70.73%。结果表明,利用人参属EST序列开发三七EST-SSR标记是可行的。
위료해인삼속식물EST중SSR분포특점급기재삼칠SSR표기중적응용,이용생물신식학방법,용primer3연건대dbEST수거고중인삼속식물인삼、서양삼화삼칠적EST서렬진행수색,발현인삼속식물EST-SSR출현빈솔위11.54%,평균매4.39kb출현1개SSR。인삼속식물단핵감산중복기원점주도지위,기차위삼핵감산중복기원화이핵감산중복기원,분별점총SSR적54.48%、17.31%화16.36%。인삼속EST-SSR적우세류형위A/T화AT/TA,분별점54.73%화8.21%。근거인삼속식물EST중적SSR설계48대인물,재합괄적PCR확증체계하,용5개삼칠양품적DNA위모판진행PCR확증,인물유효확증솔85.42%,기중다태성인물점가확증인물적70.73%。결과표명,이용인삼속EST서렬개발삼칠EST-SSR표기시가행적。
In order to know the peculiarity of EST-SSR in three species of genus Panax and using in Panax notoginseng, with bioinformatics method, the EST sequences of Panax ginseng C. A. Mey., Panax quinque foliusm L. and Panax notoginseng （Burk） F. H. Chen were searched in dbEST database by using software primer 3. The frequency of EST-SSR was 11.54%, and the average distance between SSR was 4.39 kb. Mononucleotide repeats, accounting for 54.48%, was dominant in all SSR, followed by trinucleotide and dinucleotide repeats, 7.31% and 16.36%. The most abundant motifs were A/T and AT/TA, accounted for 54.73% and 8.21%. According the EST-SSR of genus Panax to designed 48 primer pairs. Under a suitable PCR system, primers were screened against 5 DNA samples of Panax notoginseng, the effective rate of the primer amplification rate was 85.42%, and the proportion of polymorphic primers was 70.71%. The results indicated that it was feasible to development EST-SSR makers by using the EST sequences in genus Panax.