CAJ | 학술논문

以半番鸭血液基因组为模板进行PCR扩增,获得了半番鸭Agouti基因部分序列,该序列长为1178bp。序列分析表明该序列由部分第1外显子（92bp）、第1内含子（105bp）、完整的第2外显子（95bp）、完整的第2内含子（851bp）和部分第3外显子（35bp）组成;应用PCR-SSCP技术对扩增序列进一步研究发现位于第2外显子80bp处存在C/T的沉默突变,该突变的发现为进一步开展研究Agouti基因多态性与鸭羽色的相关性研究奠定基础。
이반번압혈액기인조위모판진행PCR확증,획득료반번압Agouti기인부분서렬,해서렬장위1178bp。서렬분석표명해서렬유부분제1외현자（92bp）、제1내함자（105bp）、완정적제2외현자（95bp）、완정적제2내함자（851bp）화부분제3외현자（35bp）조성;응용PCR-SSCP기술대확증서렬진일보연구발현위우제2외현자80bp처존재C/T적침묵돌변,해돌변적발현위진일보개전연구Agouti기인다태성여압우색적상관성연구전정기출。
A pair of primer was designed to amplify the sequence of Agouti gene from mule duck genomic DNA.The fragment was cloned and sequenced which was 1 178 bp in length.The sequence analysis showed this fragment consisted of partial exon 1（92 bp）,intron 1（105 bp）,exon 2 （95 bp）,intron 2 （851 bp）and partial exon 3（35 bp）.Using PCR-SSCP for this amplified fragment,a C/T silent mutant was found at 80 bp in exon 2,which is useful for the further study on polymorphism and the correlation on duck feather color.