鹌鹑原始生殖细胞的分离及冷冻
암순원시생식세포적분리급냉동
Isolation and thaw of quail PGCs
  • 万方数据
    福建畜牧兽医 복건축목수의
    FUJIAN JOURNAL OF ANIMAL HUSBANDRY AND VETERINARY
    2011年 1期 1-3 ,共3页

    张文昌%李明%庄益芬%邹长连%马群%祁喜柯%张丽

    장문창%리명%장익분%추장련%마군%기희가%장려

    鹌鹑%原始生殖细胞%分离%冷冻 鵪鶉%原始生殖細胞%分離%冷凍
    암순%원시생식세포%분리%냉동
    quail PGCs isolation thaw
    以酶分解法消化孵化了5.5d的鹌鹑性腺得到鹌鹑PGCs,比较了3种不同酶消化时间所得的细胞数量;采用2种不同的冷冻方法冻存鹌鹑PGCs。结果表明：消化15min对于鹌鹑PGCs的分离最佳,而2种冷冻程序所得的活细胞数量差异不显著。
    이매분해법소화부화료5.5d적암순성선득도암순PGCs,비교료3충불동매소화시간소득적세포수량;채용2충불동적냉동방법동존암순PGCs。결과표명：소화15min대우암순PGCs적분리최가,이2충냉동정서소득적활세포수량차이불현저。
    Incubated 5.5 days quail embryos were used to obtain cells with enzymatic digestion,and compare the number of the three ways,we thawed PGCs preserved with two methods.The results showed that the greatest number of cells after 15 minutes digested,and the results of the two methods are similar.
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