CAJ | 학술논문

采用叶绿素荧光图像分析系统，研究了野生型拟南芥（Wild Arabidopsis thaliana）Col-0不同叶龄叶片的荧光参数变化及对强光的响应。结果表明，随叶片趋于成熟或衰老，20min暗适应的最大PSⅡ光化学量子效率（Fv／Fm），稳定作用光下的PS II有效和实际光化学效率（Fv＇／Fm＇和ФPS Ⅱ）以及光化学猝灭系数（qP）均呈降低趋势，而非光化学猝灭系数（NPQ）增加；但短期5d的处理期间其变化都不明显。与低光强相比，强光处理时Fv／Fm，ФPSⅡ，Fv’／Fm’和qP呈降低趋势而NPQ明显升高。说明不同发育阶段的叶片具有明显差异的光化学反应特性，强光下光抑制现象明显；NPQ增加说明拟南芥能通过非辐射的热能耗散途径消耗过多的激发能，从而能避免光合机构的继续伤害。
채용협록소형광도상분석계통，연구료야생형의남개（Wild Arabidopsis thaliana）Col-0불동협령협편적형광삼수변화급대강광적향응。결과표명，수협편추우성숙혹쇠로，20min암괄응적최대PSⅡ광화학양자효솔（Fv／Fm），은정작용광하적PS II유효화실제광화학효솔（Fv＇／Fm＇화ФPS Ⅱ）이급광화학졸멸계수（qP）균정강저추세，이비광화학졸멸계수（NPQ）증가；단단기5d적처리기간기변화도불명현。여저광강상비，강광처리시Fv／Fm，ФPSⅡ，Fv’／Fm’화qP정강저추세이NPQ명현승고。설명불동발육계단적협편구유명현차이적광화학반응특성，강광하광억제현상명현；NPQ증가설명의남개능통과비복사적열능모산도경소모과다적격발능，종이능피면광합궤구적계속상해。
The changes of chlorophyll fluorescence parameters of different ages leaves and it responses to strong light were analyzed using CF imager in wild type Arabidopsis thaliana. The results indicated that Fv/ Fm ,ФPS II,Fv＇/Fm＇ and qP were all decreased when leaves become mature and elder,and NPQ were increased. There were no significant changes of all parameters during 5 days of development. Compared with 30 min low light treatment, after 60 min strong light irradiation, Fv/Fm, ФPS II , Fv＇/Fm＇and qP were all significantly increased and NPQ were significantly decreased. These results demonstrated that that there was significant difference among leaves of different ages and strong light could cause serious photoqnhibition. The plants can dissipate excited energy through no-radiated heat and avoid continue damage on photosynthetic function.