CAJ | 학술논문

目的为使肝前型门脉高压症动物实验更可靠，建立符合或接近其临床表现的动物模型。方法实验犬29只。开腹测脾体积、门静脉直径及压力。部分结扎门脉使其截面积缩小95.0%，门脉压上升到结扎前的3倍，胃肠无明显淤血时关腹。分别在术后2、4、8、12周及16周再次开腹观察胃肠淤血及侧支循环情况，测门脉压及脾体积。结果 4只死亡。2～8周的门脉压较高，腹膜后水肿、肠壁肥厚。随着侧支循环的建立，压力逐渐下降，12～16周门脉压稳定，腹膜后水肿消失，交通血管清晰。12、16周门脉压低于2、4周（P＜0.05）；12周与16周的门脉压力比较P>0.05。结论采用部分结扎门静脉的方法制作肝前型门脉高压症动物模型，缩窄门脉后12～16周为成功；更接近其临床表现，有一定的侧支循环的门脉高压症，此时做肝前型门脉高压症的实验数据最可靠。
목적 위사간전형문맥고압증동물실험경가고，건립부합혹접근기림상표현적동물모형。방법 실험견29지。개복측비체적、문정맥직경급압력。부분결찰문맥사기절면적축소95.0%，문맥압상승도결찰전적3배，위장무명현어혈시관복。분별재술후2、4、8、12주급16주재차개복관찰위장어혈급측지순배정황，측문맥압급비체적。결과 4지사망。2～8주적문맥압교고，복막후수종、장벽비후。수착측지순배적건립，압력축점하강，12～16주문맥압은정，복막후수종소실，교통혈관청석。12、16주문맥압저우2、4주（P＜0.05）；12주여16주적문맥압력비교P>0.05。결론 채용부분결찰문정맥적방법제작간전형문맥고압증동물모형，축착문맥후12～16주위성공；경접근기림상표현，유일정적측지순배적문맥고압증，차시주간전형문맥고압증적실험수거최가고。
Objective To insure the reliability of animal models ofpre-hepatic portal hypertension.Methods The size of spleen, diameter and pressure of portal vein of 29 canine models were measured. A partial ligation of portal vein was performed narrowing at 95% of its original cross section, or making the portal vein pressure more than three times to its original pressure and the gastrointestinal venous stasis could not be seen. The features of gastrointestinal venous stasis and collateral circulation were observed during reoperation on the 2nd, 4th, 8th, 12th and 16th weeks postoperatively.Results Four dogs died from abdominal infection, anesthesia and acute enteric necrosis respectively. The higher pressure of portal vein, retroperitoneal edema, hypertrophy of the intestinal wall were noted. Following the formation of collaterals between portal and systemic circulation , the portal vein pressure decreased gradually from 2nd to 8th weeks. From 12th to 16th weeks postoperatively, the portal vein pressure was stabilized but still higher than that of the normal, retropertioneal edema disappeared and collateral vessels could be clearly seen. The pressure of portal vein in the 12th, 16th weeks was lower than that in 2nd, 4th weeks (P<0.05). Conclusions Making animal models of prehepatic portal hypertension by narrowing the diameter of portal vein is a reliable method. The status of models in 12th～16th weeks after operation are similar to children with portal hypertension.