CAJ | 학술논문

背景与目的:甲状腺癌以淋巴道转移为主,淋巴管生成在淋巴道转移中扮演了重要角色,但其生成机制尚不明确.本研究旨在探讨甲状腺癌组织中类肝素酶(heparanase,HPSE)、VEGF-C和D2-40表达与淋巴管生成的关系.方法:用免疫组织化学SP法检测J77例(其中淋巴结转移者32例)甲状腺癌[包括乳头状癌(papillary thyroid carcinoma,PTC)、滤泡癌(follicular thyroid carcinoma,FTC)、髓样癌(medullary thyroid carcinoma,MTC)]患者中HPSE、VEGF-C与D2-40的表达水平,以D2-40标记淋巴管,在显微镜下计数淋巴管密度(lymphatic vessel density,LVD),并对不同类型的甲状腺癌分别进行相关性分析.结果:在各类甲状腺癌患者中HPSE、VEGF-C与D2-40均有不同程度的表达,其中以PTC表达最高(P<0.05);HPSE、VEGF-C与D2-40在不同类型甲状腺癌里面的表达情况分别为:PTC(54.9%、68.6%、12.8±5.7),FTC(37.5%、50%、8.6±1.7),MTC(20%、20%、4.9±0.8).HPSE、VEGF-C与D2-40的表达在有淋巴结转移组均显著高于无转移组(P值分别为0.014和0.048),且三者的表达呈正相关(r=0.616,P<0.001).结论:HPSE、VEGF-C与D2-40的表达与甲状腺癌淋巴结转移相关;HPSE和VEGF-C的表达与淋巴管生成相关.
배경여목적:갑상선암이림파도전이위주,림파관생성재림파도전이중분연료중요각색,단기생성궤제상불명학.본연구지재탐토갑상선암조직중류간소매(heparanase,HPSE)、VEGF-C화D2-40표체여림파관생성적관계.방법:용면역조직화학SP법검측J77례(기중림파결전이자32례)갑상선암[포괄유두상암(papillary thyroid carcinoma,PTC)、려포암(follicular thyroid carcinoma,FTC)、수양암(medullary thyroid carcinoma,MTC)]환자중HPSE、VEGF-C여D2-40적표체수평,이D2-40표기림파관,재현미경하계수림파관밀도(lymphatic vessel density,LVD),병대불동류형적갑상선암분별진행상관성분석.결과:재각류갑상선암환자중HPSE、VEGF-C여D2-40균유불동정도적표체,기중이PTC표체최고(P<0.05);HPSE、VEGF-C여D2-40재불동류형갑상선암리면적표체정황분별위:PTC(54.9%、68.6%、12.8±5.7),FTC(37.5%、50%、8.6±1.7),MTC(20%、20%、4.9±0.8).HPSE、VEGF-C여D2-40적표체재유림파결전이조균현저고우무전이조(P치분별위0.014화0.048),차삼자적표체정정상관(r=0.616,P<0.001).결론:HPSE、VEGF-C여D2-40적표체여갑상선암림파결전이상관;HPSE화VEGF-C적표체여림파관생성상관.
Background and purpose: Thyroid carcinoma cells spread mainly through lymph node metastasis, and lymphangiogenesis plays an important role during the lymph node metastasis, but it is not very clear to understand the formation mechanism. This study was to investigate the correlative expressions of HPSE, VEGF-C, D2-40 and lymphangiogenesis in thyroid carcinoma. Methods: Immunohistochemistry SP method was used to detect the expressions of HPSE, VEGF-C and D2-40 in 77 patients with thyroid carcinoma including papillary thyroid carcinoma (PTC), follicular thyroid carcinoma (FTC),medullary thyroid carcinoma (MTC), 32 of them with lymph node metastasis was enrolled into the study, D2-40 stained the lymphatic vessels, and lymphatic vessel density (LVD) was scanned under the light-microscope, and the correlation among the above indexes in different thyroid carcinoma types were analyzed respectively. Results: The expressions of HPSE, VEGF-C and D2-40 were observed to have a different degree in thyroid carcinoma, and the highest expression of the protein could be seen in the patients with papillary carcinoma (P<0.05),The expression ratios of HPSE,VEGF-C and D2-40 in different carcinoma types were 54.9%, 68.6%, 12.8±5.7 for PTC, 37.5%, 50%, 8.6±1.7 for FTC, 20% and 20%, 4.9±0.8 for MTC, respectively. There were significant different expressions of HPSE, VEGF-C and D2-40 between the patients with lymph node-positive group and node-negative group (P=0.014, P=0.048, respectively). In addition, the expressions of them were positively correlated (P<0.001, r=0.616). Conclusion: HPSE, VEGF-C and D2-40 have a close relationship with lymph node metastasis, HPSE and VEGF-C are related to the lymphangiogenesis.