化学工程
化學工程
화학공정
CHEMICAL ENGINEERING
2010年
1期
67-70
,共4页
何智妍%周毓婷%张海涛%苏赛男%聂华丽%朱利民
何智妍%週毓婷%張海濤%囌賽男%聶華麗%硃利民
하지연%주육정%장해도%소새남%섭화려%주이민
金属亲和膜%木瓜蛋白酶%Ni~(2+)%纯化
金屬親和膜%木瓜蛋白酶%Ni~(2+)%純化
금속친화막%목과단백매%Ni~(2+)%순화
metal affinity membrane%papain%Ni~(2+)%purification
建立了一种快速、简便的分离木瓜蛋白酶的方法.尼龙膜经壳聚糖改性后,以金属镍离子Ni~(2+)为配基制备了一种新型的蛋白质分离材料,并将该亲和膜应用于木瓜蛋白酶的分离纯化,在对洗脱液、上样速度、洗脱速度、洗脱液的pH值和洗脱液的离子强度进行优化的基础上,成功地分离纯化出木瓜蛋白酶,纯化倍数为20.59倍.
建立瞭一種快速、簡便的分離木瓜蛋白酶的方法.尼龍膜經殼聚糖改性後,以金屬鎳離子Ni~(2+)為配基製備瞭一種新型的蛋白質分離材料,併將該親和膜應用于木瓜蛋白酶的分離純化,在對洗脫液、上樣速度、洗脫速度、洗脫液的pH值和洗脫液的離子彊度進行優化的基礎上,成功地分離純化齣木瓜蛋白酶,純化倍數為20.59倍.
건립료일충쾌속、간편적분리목과단백매적방법.니룡막경각취당개성후,이금속얼리자Ni~(2+)위배기제비료일충신형적단백질분리재료,병장해친화막응용우목과단백매적분리순화,재대세탈액、상양속도、세탈속도、세탈액적pH치화세탈액적리자강도진행우화적기출상,성공지분리순화출목과단백매,순화배수위20.59배.
A shortcut and simply method was established to purify papain. The nylon membranes treated with chitosan as the affinity carrier was used to prepare a novel metal affinity membrane. Ni~(2+) as a ligand was immobilized on the metal affinity membranes. The membranes were used to separate and purify papain from crude papain powder. The conditions of purifying papain, such as elution solution, adsorption rate, desorption rate, pH of buffer and ion strength of buffer were optimized. Based on the optimum conditions, the papain is successfully purified and the purification fold is 20.59.
