CAJ | 학술논문

采用毛细管电泳-电容耦合非接触式电导(CE-C~4D),以20 mmol/L HAc + 6 mmol/L NaAc+12 mg/L羟丙基甲基纤维素(HPMC)+35 mmol/L羟丙基-β-环糊精(HP-β-CD)为电泳运行液,在熔融石英毛细管柱(45 cm×50 μm i.d.,有效长度 40 cm)中,正高压分离,手性药物氧氟沙星对映体获得良好的基线分离,线性检测范围为0.8～40 mg/L,检出限为0.3 mg/L.考察了电泳运行液组成、二元手性选择剂(HP-β-CD和HPMC)的浓度、进样方式和样品基质等对灵敏度和分离度的影响.本方法应用于市售外消旋和左旋氧氟沙星片剂中对映体的分离测定.
채용모세관전영-전용우합비접촉식전도(CE-C~4D),이20 mmol/L HAc + 6 mmol/L NaAc+12 mg/L간병기갑기섬유소(HPMC)+35 mmol/L간병기-β-배호정(HP-β-CD)위전영운행액,재용융석영모세관주(45 cm×50 μm i.d.,유효장도 40 cm)중,정고압분리,수성약물양불사성대영체획득량호적기선분리,선성검측범위위0.8～40 mg/L,검출한위0.3 mg/L.고찰료전영운행액조성、이원수성선택제(HP-β-CD화HPMC)적농도、진양방식화양품기질등대령민도화분리도적영향.본방법응용우시수외소선화좌선양불사성편제중대영체적분리측정.
Novel method of enantioseparation of ofloxacin by capillary electrophoresis with capactively coupled contactless conductivity detection (C~4D) using binary chiral selectors,hydroxypropyl cyclodextrin and hydroxypropyl methyl cellulose ( HP-β-CD and HPMC) ,was developed. The enantiomers of ofloxacin were baseline separated with an uncoated fused-silica capillary(45 cm×50 m i. d. ,L_(effi) = 40 cm)under the optimum separate conditions, 20 mmol/L HAc +6 mmol/L NaAc + 12 mg/L HPMC +35 mmol/L HP-β-CD,separation voltage +13 kV,electrokinetic injection 11 kV×10 s. The calibration curve of the enantiomers showed good linearity in the range from 0. 8 mg/L to 40 mg/L with LOD =0. 3 mg/L. Effects of several factors on resolutions,such as the composition of the running buffer,the pH value,separation voltage,injection method and the parameters of C~4D detector,were investigated. The efficacy of the HP-β-CD and HPMC were discussed. The proposed method has been successfully applied to monitor the enantiomer of ofloxacin contents in the commercial Ofloacin Tablets and Levofloxacin Hydrochloride Tablets,and was demonstrated simple,rapid and reproducible.