厦门大学学报（自然科学版）
廈門大學學報（自然科學版）
하문대학학보（자연과학판）
JOURNAL OF XIAMEN UNIVERSITY (NATURAL SCIENCE)
2009年
4期
590-593
,共4页
陆兆明%徐祯%王珂%陈澄%陈岑渌璐%宋思扬
陸兆明%徐禎%王珂%陳澄%陳岑淥璐%宋思颺
륙조명%서정%왕가%진징%진잠록로%송사양
双孢蘑菇%双向电泳%热胁迫%蛋白表达差异
雙孢蘑菇%雙嚮電泳%熱脅迫%蛋白錶達差異
쌍포마고%쌍향전영%열협박%단백표체차이
Agaricus bisporus Lange (Imbach)%2D-electrophoresis%heat stress%protein expression difference
利用蛋白质组学和荧光定量PCR方法,研究双孢蘑菇(Agaricus bisporus Lange (Imbach))02菌株在常温与高温胁迫下的蛋白质表达差异,发现了6个差异蛋白质点,经NCBI数据库比对分析,表明6个差异蛋白质分别为HSP70家族的HSS1热激蛋白、异柠檬酸裂解酶、细胞色素P450单氧化酶及3个未知蛋白质.这些蛋白质在双孢蘑菇受热胁迫下具有保护自身稳定的功能.
利用蛋白質組學和熒光定量PCR方法,研究雙孢蘑菇(Agaricus bisporus Lange (Imbach))02菌株在常溫與高溫脅迫下的蛋白質錶達差異,髮現瞭6箇差異蛋白質點,經NCBI數據庫比對分析,錶明6箇差異蛋白質分彆為HSP70傢族的HSS1熱激蛋白、異檸檬痠裂解酶、細胞色素P450單氧化酶及3箇未知蛋白質.這些蛋白質在雙孢蘑菇受熱脅迫下具有保護自身穩定的功能.
이용단백질조학화형광정량PCR방법,연구쌍포마고(Agaricus bisporus Lange (Imbach))02균주재상온여고온협박하적단백질표체차이,발현료6개차이단백질점,경NCBI수거고비대분석,표명6개차이단백질분별위HSP70가족적HSS1열격단백、이저몽산렬해매、세포색소P450단양화매급3개미지단백질.저사단백질재쌍포마고수열협박하구유보호자신은정적공능.
Agaricus bisporus is a kind of cultivated edible mushroom which has high economic value and important ecology significance.Environment temperature is the main restrained factor in its development,and this greatly limits its cultivation season. In this study,2D-eletrophoresis and real-time PCR are employed to discover the proteomic difference in Agaricus bisporus 02 strain after high temperature induction,and 6 differential expressed protein spots were finally obtained.Compared with the NCBI data base ,spot 1 is the heat shock protein ,spot 2 is isocitrate lyase and spot 3 is cytochrome P450 monooxygenase.The other three spots are novel.Presumably,those proteins confer the thermotolerance on strain 02 under high temperature.
