CAJ | 학술논문

目的探讨ATP结合盒转运子A1(ABCA1)R219K基因多态性与2型糖尿病(T2DM)合并冠心病(CHD)的关系.方法采用多聚酶链反应-限制性片段长度多态性法检测83例健康对照者(NC组)、72例单纯T2DM患者(CHD-组)、71例T2DM合并CHD患者(CHD+组)ABCA1219位点的多态基因型.结果在NC组、CHD-组、CHD+组中,KK基因型分布为19.3%、16.7%、4.2%;CHD+组与NC组、CHD-组KK基因型频率差异有统计学意义(P＜0.05).K等位基因频率分别为42.2%、38.9%、31.7%,三组间差异无统计学意义.在糖尿病患者中,KK基因型携带者的血浆HDL-C水平显著高于RR型,其差异有统计学意义(P＜0.05).结论 ABCA1R219K基因多态性可能相关于T2DM并发的CHD.
목적 탐토ATP결합합전운자A1(ABCA1)R219K기인다태성여2형당뇨병(T2DM)합병관심병(CHD)적관계.방법 채용다취매련반응-한제성편단장도다태성법검측83례건강대조자(NC조)、72례단순T2DM환자(CHD-조)、71례T2DM합병CHD환자(CHD+조)ABCA1219위점적다태기인형.결과 재NC조、CHD-조、CHD+조중,KK기인형분포위19.3%、16.7%、4.2%;CHD+조여NC조、CHD-조KK기인형빈솔차이유통계학의의(P＜0.05).K등위기인빈솔분별위42.2%、38.9%、31.7%,삼조간차이무통계학의의.재당뇨병환자중,KK기인형휴대자적혈장HDL-C수평현저고우RR형,기차이유통계학의의(P＜0.05).결론 ABCA1R219K기인다태성가능상관우T2DM병발적CHD.
Objective To investigate the relationship of the polymorphism of ATP-binding cassette transporter 1(ABCA1) R219K with coronary heart disease(CHD) in Chinese Han type 2 diabetic patients. Methods 83 normal subjects and 143 T2DM patients [71 combined with CHD (CHD+),72 without CHD(CHD-)]were enrolled in this study.The genotype and allele frequencies of ABCA1 R219K genetic variation were assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results Groups of NC,CHD-and CHD+ showed that frequencies of KK genotype were 19.3%,16.7%,4.2% separately,and the frequencies of K allele were 42.2%,38.9%,31.7% separately.The genotypes in the CHD+ group were significantly different from the NC and CHD-groups (χ2=7.441,P=0.024;χ2=9.453,P=0.009).The KK genotype had a significantly higher HDL-C level than RR and RK genotypes(all P<0.05). Conclusions The ABCA1 R219K gene polymorphism may be correlated to CHD.