CAJ | 학술논문

以批次培养为对照,研究了7种脉冲补料方式对搅拌式光生物反应器中培养大型褐藻海带配子体细胞生长和培养液内氮磷营养盐消耗的影响,并首次探讨了脉冲补料方式下不同补料时间点和补料量的影响作用.培养条件设定为50 mg DCW (细胞干重)/L接种密度、培养液为改良的APSW人工海水、光强60μE/m2.s、光周期16/8h L/D、通气速率和搅拌速率分别为50ml/min和100r/min.结果表明少量补料利于细胞对氮磷的协同吸收,进而利于生物量扩增.当培养液内氮磷富足或耗尽时补料对于生物量大量生产效果甚微,可能由于氮磷吸收变缓、其储存现象显著,或是其吸收协同性降低.当细胞生长至对数中期开始频繁补加少量氮磷营养盐,即维持培养液内氮磷浓度在各自起始浓度的1/3至1/2之间,对生物量生产最有效,生物量增长倍数高达12.270倍.
이비차배양위대조,연구료7충맥충보료방식대교반식광생물반응기중배양대형갈조해대배자체세포생장화배양액내담린영양염소모적영향,병수차탐토료맥충보료방식하불동보료시간점화보료량적영향작용.배양조건설정위50 mg DCW (세포간중)/L접충밀도、배양액위개량적APSW인공해수、광강60μE/m2.s、광주기16/8h L/D、통기속솔화교반속솔분별위50ml/min화100r/min.결과표명소량보료리우세포대담린적협동흡수,진이리우생물량확증.당배양액내담린부족혹모진시보료대우생물량대량생산효과심미,가능유우담린흡수변완、기저존현상현저,혹시기흡수협동성강저.당세포생장지대수중기개시빈번보가소량담린영양염,즉유지배양액내담린농도재각자기시농도적1/3지1/2지간,대생물량생산최유효,생물량증장배수고체12.270배.
Photoautotrophic gametophyte cells of the brown macroalgae Laminaria japonica were cultivated in 500ml stirred tank photobioreactors under seven pulse feeding modes and one batch mode.It is the first time for the study of effects of the feeding time points and feeding quantity on macroalgal cell growth and nutrient consumption.Results showed that, with inoculum density of 50mg DCW/L, in modified APSW artificial seawater medium at 13℃, light intensity of 60μE/m2.s, light cycle of 16/8h L/D, aeration rate of 50ml/min, and agitation speed of 100r/min, feeding the culture with small nutrient quantity was beneficial for the synchronization between nitrate and phosphate absorption, and further for biomass production.Feeding when ambient nutrient was abundant or depleted was quite weak for large amount of biomass accumulation, which might be due to the slowing nutrient absorption, nutrient storage, or the divergence absorption between nitrate and phosphate.Feeding nutrient frequently with small quantity from mid-exponential growth of macroalgal cells, that is maintaining medium nutrient concentration between 1/3 and 1/2 of its initial concentration, was the most effective way for biomass production, with biomass increased by 12.270 times of for 51 days' cultivation.