CAJ | 학술논문

探讨前列腺穿刺活检标本中端粒酶(TE)活性检测在前列腺癌 (PCA)诊断、鉴别诊断及评估PCA生物学行为中的意义。方法：应用端粒重复片段扩增法(TRA P法)检测疑为前列腺癌患者的前列腺穿刺活检标本的TE活性，并比较TE活性水平与PCA病理分化程度及转移情况的关系。结果：PCA组织中TE活性明显高于前列腺增生(BPH)组织。PCA 组织中TE活性水平与其病理分化程度及转移情况相关。结论：PCA患者的前列腺穿刺活检组织中的TE活性显著高于BPH者，且与其病理分化程度及转移情况相关。提示TE可能成为PCA 早期诊断及鉴别诊断的一项新的分子瘤标，并可能与PCA的生物学行为相关。
탐토전렬선천자활검표본중단립매(TE)활성검측재전렬선암 (PCA)진단、감별진단급평고PCA생물학행위중적의의。방법：응용단립중복편단확증법(TRA P법)검측의위전렬선암환자적전렬선천자활검표본적TE활성，병비교TE활성수평여PCA병리 분화정도급전이정황적관계。결과：PCA조직중TE활성명현고우전렬선증생(BPH)조직。PCA 조직중TE활성수평여기병리분화정도급전이정황상관。결론：PCA환자적전렬선천자활검 조직중적TE활성현저고우BPH자，차여기병리분화정도급전이정황상관。제시TE가능성위PCA 조기진단급감별진단적일항신적분자류표，병가능여PCA적생물학행위상관。
To verify the detection of telomerase activity in prostate needle biopsy samples could be valuable for diagnosis and e valuating the biological malignant potentials of prostate cancer.Methods :42 patient with suspected prostate cancer underwent transrectal ultraso und-guided systematic prostate needle biopsy,the prostate neelde biopsy sample w as obtained for both histological examination and telomerase assay.The telomeras e activity was assay and correlated with histological findings and status of tum or metastasis.Results:The histological examination showed 26 of 46 cases were prostate cancer,anther 16 cases belonged to benign prostatic hyper plasia.Telomerase activity was detected in 23 of 26(88.5%)prostate cancer tissue s.In contrast,only 1 of 16(6.2%) samples with benign prostatic hyperplasia was positive f or the telomerase.The result also revealed that poorly differentiated prostate cancer often expressed a high level of telomerase activity;and the tel o merase activity in the samples with tumor metastasis was higher than that withou t tumor metastasis.Conclusions:This study suggest that telomeras e activity in prostate needle biopsy samples might be a potentially useful tool for diagnosis and evaluating the biological malignant potentials of prostate can cer,although further studies are required to fully determine its prognostic pote ntial.