细胞与分子免疫学杂志
細胞與分子免疫學雜誌
세포여분자면역학잡지
2001年
2期
166-168
,共3页
万亚坤%张俊杰%陈南春%陈苏民
萬亞坤%張俊傑%陳南春%陳囌民
만아곤%장준걸%진남춘%진소민
人ERA%多克隆抗体%纯化
人ERA%多剋隆抗體%純化
인ERA%다극륭항체%순화
目的纯化兔抗人ERA的多克隆抗体。方法在大肠杆菌中，表达重组MBP-hEra融合蛋白。表达产物先继以直链淀粉树脂亲和柱和Superose12凝胶柱过滤纯化。将纯化的MBP-hEra偶联于NHS-activatedSepharoseTM上，制备亲和层析柱，纯化兔抗人ERA多克隆抗体。结果①表达、纯化的MBP-hEra，相对分子质量(Mr)为78×103，其纯度为95％。②从抗血清中纯化获得可与MBP-hEra特异性结合的兔抗hEra多克隆抗体。结论获得了特异性较好的纯化兔抗hEra多克隆抗体。
目的純化兔抗人ERA的多剋隆抗體。方法在大腸桿菌中，錶達重組MBP-hEra融閤蛋白。錶達產物先繼以直鏈澱粉樹脂親和柱和Superose12凝膠柱過濾純化。將純化的MBP-hEra偶聯于NHS-activatedSepharoseTM上，製備親和層析柱，純化兔抗人ERA多剋隆抗體。結果①錶達、純化的MBP-hEra，相對分子質量(Mr)為78×103，其純度為95％。②從抗血清中純化穫得可與MBP-hEra特異性結閤的兔抗hEra多剋隆抗體。結論穫得瞭特異性較好的純化兔抗hEra多剋隆抗體。
목적순화토항인ERA적다극륭항체。방법재대장간균중，표체중조MBP-hEra융합단백。표체산물선계이직련정분수지친화주화Superose12응효주과려순화。장순화적MBP-hEra우련우NHS-activatedSepharoseTM상，제비친화층석주，순화토항인ERA다극륭항체。결과①표체、순화적MBP-hEra，상대분자질량(Mr)위78×103，기순도위95％。②종항혈청중순화획득가여MBP-hEra특이성결합적토항hEra다극륭항체。결론획득료특이성교호적순화토항hEra다극륭항체。
Aim To purify rabbit anti-hEra polyclonal antibody. Methods Therecombinant fusion protein MBP-hEra was expressed in the E.coli and was purified through amylose resin chromatography column and Superose 12 gel fitration.The purified MBP-hEra was coupled to the NHS-activated sepharoseTM to prepare affinity chromatography column to purify rabbit anti-hEra polyclonal antibody. Results ① MBP-hEra was expressed and purified successfully with relative molecolar mass(Mr)of 78× 103and its purity could reach about 95％ .② purified Rabbit anti-hEra polyclonal antibody could bind specifically to MBP-hEra. Conclusion The purified rabbit anti-hEra polyclonal antibody has better specificity and purity.
