CAJ | 학술논문

用SUPELCOSILLC-8-DB柱为分离柱(柱温30℃)、以30:70(体积比)的甲醇：草酸铵溶液(1.25g/L)作流动相，在230nm紫外检测，反相HPLC同时快速测定了饮料和果品中的糖精、苯甲酸、山梨酸和咖啡因四种食品添加剂。在试验浓度范围2～200mg/L内均呈良好线性，它们的检出限分别为20、8、2、26ng。样品的加标回收率为104％～112％，变异系数为2.5％～4.6％(n=5)。
용SUPELCOSILLC-8-DB주위분리주(주온30℃)、이30:70(체적비)적갑순：초산안용액(1.25g/L)작류동상，재230nm자외검측，반상HPLC동시쾌속측정료음료화과품중적당정、분갑산、산리산화가배인사충식품첨가제。재시험농도범위2～200mg/L내균정량호선성，타문적검출한분별위20、8、2、26ng。양품적가표회수솔위104％～112％，변이계수위2.5％～4.6％(n=5)。
A simple,rapid method for simultaneous determination of four foodadditives,that is, saccharin, benzoic acid,sorbic acid and caffeine in beverages and foods by RP-HPLC has been described. The separation and determination of those four compounds were based on a SUPELCOSIL LC-8-DB column with UV detection at 230nm and methanol-1.25g/ L ammonium oxalate solution (30:70,v:v) as mobile phase. The relationship of the linearity method was proven to be acceptable and the detection limits for those four compounds were 20,8,2,26ng,respectively. The spike recoveries for the determination were in the ranges of 104%～ 112% and the coefficients of variation below 4.6%(n=5).