CAJ | 학술논문

采用蒙古沙土鼠双侧颈总动脉阻断前脑缺血模型, 以放射自显影（反向磷酸化, back-phosphorylation）及免疫印迹(Western blotting)法体外测定缺血时纹状体DARPP-32磷酸化水平和蛋白含量的变化。结果表明, 短暂性缺血纹状体DARPP-32的免疫学活性和蛋白含量无明显改变。在缺血10 min内, 随缺血时间的延长, 体外DARPP-32的［32P］的掺入量在缺血5 min时升高, 在缺血2、7、10 min时均降低, 而反向磷酸化的测定结果表明体内DARPP-32磷酸化水平增高, 说明缺血可诱导DARPP-32磷酸化水平变化。
채용몽고사토서쌍측경총동맥조단전뇌결혈모형, 이방사자현영（반향린산화, back-phosphorylation）급면역인적(Western blotting)법체외측정결혈시문상체DARPP-32린산화수평화단백함량적변화。결과표명, 단잠성결혈문상체DARPP-32적면역학활성화단백함량무명현개변。재결혈10 min내, 수결혈시간적연장, 체외DARPP-32적［32P］적참입량재결혈5 min시승고, 재결혈2、7、10 min시균강저, 이반향린산화적측정결과표명체내DARPP-32린산화수평증고, 설명결혈가유도DARPP-32린산화수평변화。
Using the mongolian gerbil model of transient forebrain ischemia by bilateral carotid arteries occlusion, the levels of phosphorylation and the amount of DARPP-32 in striatum were measured by back-phosphorylation and Western-blotting assay in vitro. Transient forebrain ischemia had no effect on the immunoreaction of DARPP-32 in striatum, but the state of phosphorylation of DARPP-32 showed a significant change. The ［32P］ phosphate incorporation in DARPP-32 decreased after 2, 7 or 10 min of ischemia, but increased after a 5-min ischemia. In contrast, the result of back-phosphorylation in vivo was opposite to that obtained in vitro.