CAJ | 학술논문

本文采用100％二甲基甲酰胺(DMF)作为样品的溶剂，以Tris-硼砂作为分离甜菊糖苷的缓冲体系，探讨了进样量、柱温、电压、Tris-硼砂缓冲液浓度以及pH值等条件对甜菊糖苷分离结果的影响。通过优化各种分离条件，实现了毛细管电泳高效分离测定甜菊糖苷。
본문채용100％이갑기갑선알(DMF)작위양품적용제，이Tris-붕사작위분리첨국당감적완충체계，탐토료진양량、주온、전압、Tris-붕사완충액농도이급pH치등조건대첨국당감분리결과적영향。통과우화각충분리조건，실현료모세관전영고효분리측정첨국당감。
This paper reported a new, effective and secure method for separation and determination of stevioside by capillary electrophoresis. The effects of di{ferent separation conditions on the migration time, selectivity and resolution were investigated. Under optimum separation conditions, the detector response for stevioside was linear over the range 0.05－9 mg/ml and the linear regression coefficient was 0.997. All components provided good resolution in 3.5 min. The relative standard deviations for the migration time of St and RA were 0.71％ and 0.46％, respectively. This method was applied to the determination of steviol extract, and the content of St in this sample was 5.36％.