CAJ | 학술논문

石远321是从海岛棉×野生色伯氏棉×陆地棉三元杂种后代中选育而成的种间杂交新品种。其细胞学鉴定结果、形态学基本特征以及栽培特性均为陆地棉型。但在丰产性、棉纤维品质及抗逆性等许多农艺性状和经济性状方面，该品种明显综合了多棉种多亲本的遗传特性。为了从分子水平上认识石远321的三元杂种性质及其变异水平，用等电聚焦电泳和RAPD技术，对石远321及其不同棉种亲本进行了遗传分析。主要结果如下：（1）酯酶同工酶图谱分析表明，石远321与其野生棉亲本和海岛棉亲本同具有一条陆地棉亲本所没有的特异带；（2）用4个引物对石远321扩增共发现6个野生棉亲本和海岛棉亲本的特征片段；（3）通过比较石远321与其3个不同棉种亲本之间的RAPD差异，说明石远321在基因组水平上具有高度的遗传异质性。初步从分子水平上证实了石远321为上述3个棉种的种间杂交后代。
석원321시종해도면×야생색백씨면×륙지면삼원잡충후대중선육이성적충간잡교신품충。기세포학감정결과、형태학기본특정이급재배특성균위륙지면형。단재봉산성、면섬유품 질급항역성등허다농예성상화경제성상방면，해품충명현종합료다면충다친본적유전특성。위료종분자수평상인식석원321적삼원잡충성질급기변이수평，용등전취초전영화RAPD기술，대석원321급기불동면충친본진행료유전분석。주요결과여하：（1）지매동공매도보분석표명，석원321여기야생면친본화해도면친본동구유일조륙지면친본소몰유적특이대；（2）용4개인물대석원321확증공발현6개야생면친본화해도면친본적특정편단；（3）통과비교석원321여기3개불동면충친본지간적RAPD차이，설명석원321재기인조수평상구유고도적유전이질성。 초보종분자수평상증실료석원321위상술3개면충적충간잡교후대。
To study the genetic character of trispecific hybrid cultivar Shiyuan 321, we analyzed this cultivar and its parents by isozyme and RAPD techniques. The main results are as follows: (1) Esterase isozyme analysis showed that there was one specific band among cultivar Suiyuan 321 and its parents, G. thurberi and G. barbadense; (2) Six specific DNA fragments amplified by 4 primers were found among cultivar Shiyuan 321 and its parents G. barbadense, G. thurberi; (3) By comparing RAPD differences among Shiyuan 321 and its parents, it is indicated that the genetic heterogeneity of cultivar Shiyuan 321 is very high at its genomic DNA level. The above results proved that Shiyuan 321 is a trispecific cultivar from G. barbadense×G. thurberi×G. hirsutum as well as a new germplasm.