CAJ | 학술논문

利用顺序基因组-重复序列原位杂交技术对1个来自中3不育系和普通小麦恢75杂种后代稳定株系H96276-2的染色体组成进行了分析。以中间偃麦草（Agropyronintermedium）基因组DNA为探针的荧光原位杂交结果表明，H96276-2的体细胞中有42条染色体，包括20对小麦染色体和1对小麦-中间偃麦草易位染色体，中间偃麦草染色体的易位片段位于1对小麦染色体的端部。进而用重复序列探针pSc119进行第2次荧光原位杂交，证明H96276-2中的中间偃麦草染色体易位片段位于小麦2B染色体的短臂上。
이용순서기인조-중복서렬원위잡교기술대1개래자중3불육계화보통소맥회75잡충후대은정주계H96276-2적염색체조성진행료분석。이중간언맥초（Agropyronintermedium）기인조DNA위탐침적형광원위잡교결과표명，H96276-2적체세포중유42조염색체，포괄20대소맥염색체화1대소맥-중간언맥초역위염색체，중간언맥초염색체적역위편단위우1대소맥염색체적단부。진이용중복서렬탐침pSc119진행제2차형광원위잡교，증명H96276-2중적중간언맥초염색체역위편단위우소맥2B염색체적단비상。
The chromosome constitution on H96276-2, which is a stable line derived from a sterile wheat-Agropyron intermedium partial amphiploid Zhong 3 cross to common wheat Hui 75, was analyzed by sequential GISH and FISH. The results of GISH using genomic DNA of Agropyron intermedium as probe showed that H96276-2 possessed 20 pairs of wheat chromosomes and one pair of translocation chromosomes of wheat and Ag. intermedium. The translocated alien chromosome fragments located on the terminals of one pair of wheat chromosomes. Furthermore, reprobing of repeat sequence pSc119 was used to identify the wheat chromosomes involved in the translocation. It was determined that the alien fragments located in the terminals of the short arms of 2B chromosomes of wheat.