CAJ | 학술논문

研究CCK基因在不同日龄大鼠脑中转录水平上的表达。取出生后不同日龄Wistar大鼠脑组织，提取总RNA，甲醛凝胶电泳，Northern印迹与α-32P标记CCK cDNA的探针杂交，放射自显影后，经激光扫描测定自显影图中斑点光密度，以估量CCK mRNA表达的相对水平。结果表明，刚出生的大鼠脑中CCK的mRNA含量甚低，随着鼠龄增长，浓度增高，20日龄时CCK mRNA浓度急剧升高，40日龄CCK mRNA的水平稍降低。CCK基因在转录水平的表达与个体发育有关。
연구CCK기인재불동일령대서뇌중전록수평상적표체。취출생후불동일령Wistar대서뇌조직，제취총RNA，갑철응효전영，Northern인적여α-32P표기CCK cDNA적탐침잡교，방사자현영후，경격광소묘측정자현영도중반점광밀도，이고량CCK mRNA표체적상대수평。결과표명，강출생적대서뇌중CCK적mRNA함량심저，수착서령증장，농도증고，20일령시CCK mRNA농도급극승고，40일령CCK mRNA적수평초강저。CCK기인재전록수평적표체여개체발육유관。
In this paper the clone was used as probe to study its expression for revealing the relationship between the level of CCK mRNA and the brain development.Total RNA from Wistar rats of various stages of development was isolated by acid guanidinium-thiocyanate phenol-chloroform extraction,followed by formaldehyde gel-electrophoresis.Northern blot,hybridization with a32P-labeled CCK cDNA probe,autoradiography and quantitation were performed by the laser density scanning.It was concluded from the results that the quantites of CCK mRNA in rat brain increased during development.From those mentioned above,it can be said that brain CCK mRNA may serve as a marker for the brain development