CAJ | 학술논문

文昌鱼是从无脊椎动物到脊椎动物的过渡类型，在进化上占有极重要的地位。本文以L-多巴为底物，显示酚氧化酶(PO)存在于文昌鱼体表上皮细胞的胞质中，PO颗粒呈圆形均质状，直径在420～700 nm之间，电子密度较高，其外无膜包被。苯硫尿(PTU)抑制酚氧化酶反应，指出该反应为PO的特异性反应，而不是底物的自氧化或其它假阳性反应。
문창어시종무척추동물도척추동물적과도류형，재진화상점유겁중요적지위。본 문이L-다파위저물，현시분양화매(PO)존재우문창어체표상피세포적포질중，PO과립정원 형균질상，직경재420～700 nm지간，전자밀도교고，기외무막포피。분류뇨(PTU)억제분 양화매반응，지출해반응위PO적특이성반응，이불시저물적자양화혹기타가양성반응。
Phenoloxidase (PO) has been shown to be present in both invertebrates and vertebrates, yet little is known about PO in Branchiostoma belcheri tsingt auense, the lancelet or amphioxus, a cephalochordate transitional from inverte brat es to vertebrates. It was shown in the present study that PO existed in the epid ermal cells of the animal. Ultrastructural examination revealed that phenoloxida se reaction products, were located in the cytoplasm of epidermal cells as an hom ogenous, electron-dense masses. This is the first report on ultrastructural loc a lization of PO in amphioxus. PO could serve as protecting agents against environ mental pathogens and foreign bodies.