CAJ | 학술논문

目的：在建立体外多药抗药细胞株的基础上，研究其抗药性形成机制，寻求克服抗药性的方法。方法：用人食管癌上皮细胞Eea-109细胞，在无诱导剂的情况下，以递加培养液中阿霉素(ADM)质量浓度的方法，建立了Eca-109／ADM多药抗药细胞株。用荧光光度法测定抗药细胞内还原性谷胱甘肽(GSH)含量及ADM的累积量。结果：研究发现Eca-109／ADM细胞内GSH含量增加、降低细胞内GSH的含量，细胞的抗药性呈现部分逆转；抗药细胞内ADM的质量浓度较敏感株Eca-109细胞低，用维拉帕米能增加抗药细胞内ADM的质量浓度，同时部分逆转其抗药性。结论：Eca-109／ADM细胞的抗药机制主要是由于细胞内GSH含量升高及细胞内药物质量浓度降低所致。
목적：재건입체외다약항약세포주적기출상，연구기항약성형성궤제，심구극복항약성적방법。방법：용인식관암상피세포Eea-109세포，재무유도제적정황하，이체가배양액중아매소(ADM)질량농도적방법，건립료Eca-109／ADM다약항약세포주。용형광광도법측정항약세포내환원성곡광감태(GSH)함량급ADM적루적량。결과：연구발현Eca-109／ADM세포내GSH함량증가、강저세포내GSH적함량，세포적항약성정현부분역전；항약세포내ADM적질량농도교민감주Eca-109세포저，용유랍파미능증가항약세포내ADM적질량농도，동시부분역전기항약성。결론：Eca-109／ADM세포적항약궤제주요시유우세포내GSH함량승고급세포내약물질량농도강저소치。
Aim: In order to investigate the mechanism of multidrug-resistance in Eca-109/ADM cell line and find ways toreverse resistance to drugs. Methods: Using a multidrug-resistant cell line of Eca-109/ADM established by stepwise selection onexposure to increasing dose of Adriamycin(ADM) in the absence of mutagenic agents, GSH content and ADM accumulation in cellswere determined. Results: GSH content in Eca-109 and Eca-109/ADM cells are (0.32±0.05), (0.69±0.09)ng/106 cells re-spectively. At lowering intracellular GSH content, the drug resistance was only overcome partially. Intracellular ADM accumulationin Eca-109/ADM cells was lower than that in Eca-109 cell. Vempamil in combination with ADM was found to enhance intracellularADM accumulation in Eca-109/ADM cells and brought about the reversion of drug resistance. Conclusion: The mechanism ofEca-109/ADM cell resistance to multiple drug results mainly from increased GSH content and decreased the drug accumulation incells.