CAJ | 학술논문

【目的】探讨采用植物组织培养技术进行中国芦荟的快速繁殖，为工厂化育苗提供技术依据。【方法】以中国芦荟地下茎发生的幼芽为外植体，诱导丛生芽的产生。【结果】基本培养基选择MT(MurashigeandTucker)较好；培养基中附加6-苄基氨基嘌呤(6-Benzylaminopurine,BA)能提高成芽率，并能增加外植体出芽的数量；BA浓度为2mg·L-1时，有利于芽的诱导与生长，出芽率达100%；根的诱导，以MT+0.5mg·L-1萘乙酸(Naphthaleneacid,NAA)培养基较好，生根率为100%。【结论】通过培养芦荟地下茎发生的幼芽，既可以迅速建立快速繁殖体系，又能使优良品种的种性得以保持。
【목적】탐토채용식물조직배양기술진행중국호회적쾌속번식，위공엄화육묘제공기술의거。【방법】이중국호회지하경발생적유아위외식체，유도총생아적산생。【결과】기본배양기선택MT(MurashigeandTucker)교호；배양기중부가6-변기안기표령(6-Benzylaminopurine,BA)능제고성아솔，병능증가외식체출아적수량；BA농도위2mg·L-1시，유리우아적유도여생장，출아솔체100%；근적유도，이MT+0.5mg·L-1내을산(Naphthaleneacid,NAA)배양기교호，생근솔위100%。【결론】통과배양호회지하경발생적유아，기가이신속건립쾌속번식체계，우능사우량품충적충성득이보지。
【Objective】To find out technological evidence for the rapid propagation of Aloe vera L.var.chinensis (Haw.)Berber.【 Methods】The technique of pl ant tissue culture was used to study the rapid propagation of Aloe vera L.Var.Chinesis(Haw.)Berger.The shoot growing from the underground stem of Aloe vera L.V ar.chinensis(Haw.)Berger was taken as the explant to induce fascic ular bud.【Res ults】The optimal culture medium was MT.MT culture medium added with 6-benzylami nopurine(BA) was effe ctive to induce the shoot formation and increase the direct shooting rate.The op timal BA concentration for the induction and formation of shoot was 2 mg·L-1,the shooting rate being 100%.As for the induction of rooting,MT culture medi um adding with 0.5 mg·L-1naphthalene acid was optimal,the rooting rate being 100%.【Conc lusion】The culture of Aloe shoot growing from the underground stem,prop er culture medium and BA concentration are important for the rapid propagation of Aloe.