CAJ | 학술논문

研究了中药黄芩(Scalellaria baicalensis Georgi)的微繁技术。TDZ[N-phenyl-N'-(1,2,3-thidiazol-5-ylurea)]用于组培中能有效地诱导黄芩完整实生苗、白化下胚轴段和茎段再生新枝。下胚轴段和茎段外植体的组织学研究显示其再生是通过愈伤组织形成新枝。TDZ诱导黄芩3种组培外植体枝再生的比较表明，理想的再生不一定要外植体脱离母体。黄芩完整实生苗的下胚轴上形成的枝明显多于下胚轴段上形成的枝，表明完整实生苗中产生的代谢产物提供枝再生所需的物质。95％以上的再生体形成根系，在无菌培养和温室中可形成完整植株。本研究所采用的再生程序可以用于这种药用植物的改良和大量繁殖
연구료중약황금(Scalellaria baicalensis Georgi)적미번기술。TDZ[N-phenyl-N'-(1,2,3-thidiazol-5-ylurea)]용우조배중능유효지유도황금완정실생묘、백화하배축단화경단재생신지。하배축단화경단외식체적조직학연구현시기재생시통과유상조직형성신지。TDZ유도황금3충조배외식체지재생적비교표명，이상적재생불일정요외식체탈리모체。황금완정실생묘적하배축상형성적지명현다우하배축단상형성적지，표명완정실생묘중산생적대사산물제공지재생소수적물질。95％이상적재생체형성근계，재무균배양화온실중가형성완정식주。본연구소채용적재생정서가이용우저충약용식물적개량화대량번식
The development of an efficient micropropagation system for Huang qin (Sculellaria baicalensis Georgi), a traditional Chinese medicinal plant used in the treatment of a wide range of human ailments, is described. Thidiazuron [TDZ: N-phenyl-N'-(1, 2, 3-thidiazol-5-ylurea)] effectively induced regeneration on cultured intact seedlings, etiolated hypocotyl explants and sterile stem segments of Huang-qin. Histological examinations of excised hypocotyls or nodal ex plants revealed that the regenerants were primarily shoots formed de novo on an intermediate callus. The comparison of the rates of TDZ-induced regeneration in the three tissue types indicates that the isolation of the explants was not essential for optimal regenerative efficiency. Significantly more regenerants formed on the hypocotyls of intact seedlings (20shoots/explant) than were observed on the excised hypocotyls (9.7shoots/explant) indicating that endogenous metabolites produced in adjacent tissues provided resources for the shoot initiation. More than 95% of de novo regenerants formed roots and then intact plantlets under either sterile culture or greenhouse conditions. The regeneration protocols developed in this study may provide the basis for the improvement and mass micropropagation of this crop.