CAJ | 학술논문

以前从菜花烙铁头蛇毒中分离纯化到Jerdonitin. 与其他Ⅱ型蛇毒金属蛋白酶相比, Jerdonitin由金属蛋白酶和去整合素两个结构域组成. 但没有检测到其出血和纤维蛋白原降解活性, 推测可能高压液相色谱的有机溶液影响了其酶活性. 采用不含高压液相色谱柱层析的新分离手段分离得到Jerdonitin. Jerdonitin在还原和非还原SDS-PAGE电泳中分别呈现一条表观分子量为38和36 kDa的条带. 像其他典型的蛇毒金属蛋白酶一样, Jerdonitin优先降解人纤维蛋白原的alpha链, 并且该活性能被EDTA完全抑制, 而PMSF对其没有影响. Jerdonitin不诱导小白鼠皮下出血.
이전종채화락철두사독중분리순화도Jerdonitin. 여기타Ⅱ형사독금속단백매상비, Jerdonitin유금속단백매화거정합소량개결구역조성. 단몰유검측도기출혈화섬유단백원강해활성, 추측가능고압액상색보적유궤용액영향료기매활성. 채용불함고압액상색보주층석적신분리수단분리득도Jerdonitin. Jerdonitin재환원화비환원SDS-PAGE전영중분별정현일조표관분자량위38화36 kDa적조대. 상기타전형적사독금속단백매일양, Jerdonitin우선강해인섬유단백원적alpha련, 병차해활성능피EDTA완전억제, 이PMSF대기몰유영향. Jerdonitin불유도소백서피하출혈.
Previously, we have purified Jerdonitin from Trimeresurus jerdonii venom. Compared with other P-Ⅱ class snake venom metalloproteinases (SVMPs), Jerdonitin has a primary structure comprising metalloproteinase and disintegrin domains. However, no hemorrhagic and fibrinogenolytic activities were detected for Jerdonitin. We thought that organic buffer of high performance liquid charamatography (HPLC) might affect its enzymatic activity. In this study, we purified Jerdonitin by another procedure excluding the HPLC. It was homogenous as judged by SDS-PAGE and had an apparent molecular weight of 36 kDa under non-reducing conditions and 38 kDa under reducing conditions, respectively. Like other typical metalloproteinases, Jerdonitin preferentially degraded alpha-chain of human fibrinogen and this fibrinogenolytic activity was completely inhibited by EDTA, but not by PMSF. It was interesting that Jerdonitin did not induce hemorrhage after intradermal injection in mice.