CAJ | 학술논문

水稻苗期冷害是导致水稻减产的重要因素之一.为了发掘新的耐冷基因、诠释其耐冷分子机制并应用分子育种的手段提高水稻苗期耐冷性,前期已通过Northern表达分析,从已构建的水稻苗期低温诱导表达正向抑制差减cDNA文库中,发现了一个受低温诱导上调表达的功能未知的C_2H_2型锌指结构域蛋白新基因(特命名为OsCOI).本研究进一步通过RT-PCR的方法从水稻中克隆了该基因,构建了其植物过表达载体,并通过农杆菌介导转入水稻基因组中,获得了其过表达转基因水稻株系,为进一步研究该基因的功能并探讨其在水稻耐冷分子育种中的应用价值奠定了基础.
수도묘기냉해시도치수도감산적중요인소지일.위료발굴신적내랭기인、전석기내랭분자궤제병응용분자육충적수단제고수도묘기내랭성,전기이통과Northern표체분석,종이구건적수도묘기저온유도표체정향억제차감cDNA문고중,발현료일개수저온유도상조표체적공능미지적C_2H_2형자지결구역단백신기인(특명명위OsCOI).본연구진일보통과RT-PCR적방법종수도중극륭료해기인,구건료기식물과표체재체,병통과농간균개도전입수도기인조중,획득료기과표체전기인수도주계,위진일보연구해기인적공능병탐토기재수도내랭분자육충중적응용개치전정료기출.
Chilling injure in seedling period is one of major limiting factors for productivity of rice(Oryza sativa L.).To improve rice cold tolerance in seedling period,it is important to exploit novel cold-resistant genes,unravel the underlying molecular mechanism and utilize the genes in cold-resistant molecular breeding. A novel cold-inducible gene encoding a putative C_2H_2-type zinc finger protein,designated OsCOI(Oryza sativa Cold-Inducible),have been identified in our former research through northern blot analysis.In this study,the gene was cloned to a plant binary over-expression vector AHLG and transformed into rice by Agrobacterium-mediated approach.Finally,through PCR screening method,some over-expression transgenic rice lines were acquired.These works established a basis for further studying the function and application potential of the gene in rice seedling cold tolerance.