广东化工
廣東化工
엄동화공
GUANGDONG CHEMICAL INDUSTRY
2012年
2期
258-260
,共3页
燃料乙醇%中试生产%玉米芯%稀酸预处理%分步水解与发酵
燃料乙醇%中試生產%玉米芯%稀痠預處理%分步水解與髮酵
연료을순%중시생산%옥미심%희산예처리%분보수해여발효
fuel ethanol production%pilot scale%corncob%dilute acid pretreatment%separated saccharification and fermentation（SHF）
利用正交试验在中试水平考察了玉米芯的稀硫酸预处理和分步糖化与水解生产乙醇的工艺。结果：最佳预处理工艺为稀硫酸浓度1.1%,温度120℃,固液比1∶8,时间3 h;酶解糖化最佳工艺为：起始底物浓度180 g/L,滤纸酶活：纤维二糖酶活=20 IU/g底物：7 IU/g底物,pH=5.0,48 h;利用运动发酵单胞菌发酵酶解液,35℃,48 h,发酵液中乙醇浓度最高67.8 g/L。
利用正交試驗在中試水平攷察瞭玉米芯的稀硫痠預處理和分步糖化與水解生產乙醇的工藝。結果：最佳預處理工藝為稀硫痠濃度1.1%,溫度120℃,固液比1∶8,時間3 h;酶解糖化最佳工藝為：起始底物濃度180 g/L,濾紙酶活：纖維二糖酶活=20 IU/g底物：7 IU/g底物,pH=5.0,48 h;利用運動髮酵單胞菌髮酵酶解液,35℃,48 h,髮酵液中乙醇濃度最高67.8 g/L。
이용정교시험재중시수평고찰료옥미심적희류산예처리화분보당화여수해생산을순적공예。결과：최가예처리공예위희류산농도1.1%,온도120℃,고액비1∶8,시간3 h;매해당화최가공예위：기시저물농도180 g/L,려지매활：섬유이당매활=20 IU/g저물：7 IU/g저물,pH=5.0,48 h;이용운동발효단포균발효매해액,35℃,48 h,발효액중을순농도최고67.8 g/L。
The goal of this study is to achieve the optimum process parameters for fuel ethanol production at pilot scale from corncob feedstock.After the optimization of orthogonal experiment,the process configuration including dilute acid pretreatment and separated saccharification and fermentation（SHF） has been obtained： the optimum parameters of pretreatment process are at 120 ℃ for 3 h with 1.1 % sulphuric acid as well as solid-to-liquid ratio=1：8,and the optimum condition of enzymatic hydrolysis are at initial pH 5.0 and 48 ℃ for 48 h with FPA： CBA=20： 7（IU/g substrate）.The maximum ethanol consistency in the fermentation broth of enzymatic hydrolyzates by Zymomonas mobilis at 35 ℃ for 48 h is 67.8 g/L from 180 g/L substrate produced by pretreatment.
