中华临床营养杂志
中華臨床營養雜誌
중화림상영양잡지
CHINESE JOURNAL OF CLINICAL NUTRITION
2011年
6期
400-403
,共4页
殷宗宝%邓超%曹玉芳%陈栩栩
慇宗寶%鄧超%曹玉芳%陳栩栩
은종보%산초%조옥방%진허허
肺纤维化%中/长链脂肪乳%ω-3鱼油脂肪乳%干扰素γ%转化生长因子β1%白细胞介素4
肺纖維化%中/長鏈脂肪乳%ω-3魚油脂肪乳%榦擾素γ%轉化生長因子β1%白細胞介素4
폐섬유화%중/장련지방유%ω-3어유지방유%간우소γ%전화생장인자β1%백세포개소4
Pulmonary fibrosis%Middle/light fatty acids%ω-3 fatty acids%Interferon garmma%Transforming growth factor β1%Interleukin-4
目的 探讨ω-3鱼油脂肪乳和20％中/长链脂肪乳注射液干预博莱霉素致肺纤维化大鼠的不同效果.方法 将清洁级雄性大鼠120只,完全随机分成生理盐水正常组、博莱霉素致大鼠肺纤维化模型对照组、博莱霉素致大鼠肺纤维化+20％中/长链脂肪乳注射液(LCT组)和博莱霉素致大鼠肺纤维化+ ω-3鱼油脂肪乳(ω-3PUFA组).在第7、14、21天分别取左肺下叶进行免疫组织化学检测转化生长因子β1(TGF-β1)和干扰素γ(IFN-γ)的表达,右肺下叶进行HE染色病理观察；采用ELISA双抗体夹心法测定各组大鼠血清白细胞介素4 (IL-4)和IFN-γ的含量.结果 ω-3PUFA组大鼠肺泡炎、肺纤维化程度较对照组和LCT组轻；对照组和LCT组大鼠在第7、14、21天的肺组织TGF-β1染色呈强阳性,IFN-γ染色较淡,而ω-3PUFA组TGF-β1和IFN-γ染色则相反,第7、14、21天,TGF-β1定量表达( 13.60±5.90、10.53±4.21、7.23±2.21)逐渐减少(P=0.047),IFN-γ定量表达(13.85 ±7.48、15.32 ±2.12、18.74±2.65)逐渐增加(P=0.041).对照组和LCT组大鼠第7、14、21天血清IL-4含量较高,IFN-γ含量较低；而ω-3PUFA组则相反(P =0.008),ω-3PUFA组第7、14、21天IL-4含量[(8.73±1.20)、(5.73±2.03)、(4.98±1.89) pg/ml]逐渐减低(P=0.044),IFN-γ含量[(5.67±0.13)、(6.58±0.64)、(7.05±0.52) pg/ml]逐渐增加(P=0.048).结论 ω-3鱼油脂肪乳可以下调大鼠肺组织TGF-β1,降低其血清含量和IL-4含量；上调肺组织IFN-γ的表达,增加其血清含量,在小动物中减轻肺纤维化优于中/长链脂肪乳注射液.
目的 探討ω-3魚油脂肪乳和20％中/長鏈脂肪乳註射液榦預博萊黴素緻肺纖維化大鼠的不同效果.方法 將清潔級雄性大鼠120隻,完全隨機分成生理鹽水正常組、博萊黴素緻大鼠肺纖維化模型對照組、博萊黴素緻大鼠肺纖維化+20％中/長鏈脂肪乳註射液(LCT組)和博萊黴素緻大鼠肺纖維化+ ω-3魚油脂肪乳(ω-3PUFA組).在第7、14、21天分彆取左肺下葉進行免疫組織化學檢測轉化生長因子β1(TGF-β1)和榦擾素γ(IFN-γ)的錶達,右肺下葉進行HE染色病理觀察；採用ELISA雙抗體夾心法測定各組大鼠血清白細胞介素4 (IL-4)和IFN-γ的含量.結果 ω-3PUFA組大鼠肺泡炎、肺纖維化程度較對照組和LCT組輕；對照組和LCT組大鼠在第7、14、21天的肺組織TGF-β1染色呈彊暘性,IFN-γ染色較淡,而ω-3PUFA組TGF-β1和IFN-γ染色則相反,第7、14、21天,TGF-β1定量錶達( 13.60±5.90、10.53±4.21、7.23±2.21)逐漸減少(P=0.047),IFN-γ定量錶達(13.85 ±7.48、15.32 ±2.12、18.74±2.65)逐漸增加(P=0.041).對照組和LCT組大鼠第7、14、21天血清IL-4含量較高,IFN-γ含量較低；而ω-3PUFA組則相反(P =0.008),ω-3PUFA組第7、14、21天IL-4含量[(8.73±1.20)、(5.73±2.03)、(4.98±1.89) pg/ml]逐漸減低(P=0.044),IFN-γ含量[(5.67±0.13)、(6.58±0.64)、(7.05±0.52) pg/ml]逐漸增加(P=0.048).結論 ω-3魚油脂肪乳可以下調大鼠肺組織TGF-β1,降低其血清含量和IL-4含量；上調肺組織IFN-γ的錶達,增加其血清含量,在小動物中減輕肺纖維化優于中/長鏈脂肪乳註射液.
목적 탐토ω-3어유지방유화20％중/장련지방유주사액간예박래매소치폐섬유화대서적불동효과.방법 장청길급웅성대서120지,완전수궤분성생리염수정상조、박래매소치대서폐섬유화모형대조조、박래매소치대서폐섬유화+20％중/장련지방유주사액(LCT조)화박래매소치대서폐섬유화+ ω-3어유지방유(ω-3PUFA조).재제7、14、21천분별취좌폐하협진행면역조직화학검측전화생장인자β1(TGF-β1)화간우소γ(IFN-γ)적표체,우폐하협진행HE염색병리관찰；채용ELISA쌍항체협심법측정각조대서혈청백세포개소4 (IL-4)화IFN-γ적함량.결과 ω-3PUFA조대서폐포염、폐섬유화정도교대조조화LCT조경；대조조화LCT조대서재제7、14、21천적폐조직TGF-β1염색정강양성,IFN-γ염색교담,이ω-3PUFA조TGF-β1화IFN-γ염색칙상반,제7、14、21천,TGF-β1정량표체( 13.60±5.90、10.53±4.21、7.23±2.21)축점감소(P=0.047),IFN-γ정량표체(13.85 ±7.48、15.32 ±2.12、18.74±2.65)축점증가(P=0.041).대조조화LCT조대서제7、14、21천혈청IL-4함량교고,IFN-γ함량교저；이ω-3PUFA조칙상반(P =0.008),ω-3PUFA조제7、14、21천IL-4함량[(8.73±1.20)、(5.73±2.03)、(4.98±1.89) pg/ml]축점감저(P=0.044),IFN-γ함량[(5.67±0.13)、(6.58±0.64)、(7.05±0.52) pg/ml]축점증가(P=0.048).결론 ω-3어유지방유가이하조대서폐조직TGF-β1,강저기혈청함량화IL-4함량；상조폐조직IFN-γ적표체,증가기혈청함량,재소동물중감경폐섬유화우우중/장련지방유주사액.
Objective To compare the effectiveness of ω-3 fatty acids and middle/light fatty acids in the intervention of rats model of bleomycin-induced pulmonary fibrosis.Methods Totally 120 rats were randomly divided into 4 groups: normal saline (NS) group,bleomycin-induced pulmonary fibrosis without treatment group ( BLM group),middle/light fatty acids group,ω-3 fatty acids group.Lung tissues were obtained on the 7th,14th,and 21st day after modeling.The left lung were measured by using immunohistochemical methods for transforming growth factor β1 (TGF-β1) and interferon garmma ( IFN-γ).The lower lobe of the right lung underwent HE staining.Serum TGF-β1,IFN-γ,and interleukin-4 (IL-4) were measured using double-antibody sandwich ELISA.Results The pulmonary alveolitis and fibrosis in the ω-3 fatty acids group was significantly milder than in middle/light fatty acids group and BLM group.On the 7th,14th,and 21st day after modeling,stronger TGF-β1 protein expression was detected in the bronehiolar epithelia of middle/light fatty acids group and BLM group and poorer IFN-γexpression in both groups.However,the opposite results were found in the ω-3 fatty acids group: on the 7th,14th,and 21st day after modeling,TGF-β1 protein expression ( 13.60 ± 5.90,10.53 ± 4.21,and 7.23 ± 2.21 )was lower ( P =0.047) and IFN-γ ( 13.85 ± 7.48,15.32 ± 2.12,and 18.74 ± 2.65 ) was stronger in ω-3 fatty acids group (P =0.041).On the 7 ,14,and 21st day after modeling,the serum IL-4 levels in the middle/light fatty acids group and BLM group became significantly higher,while the IFN-γ level in both groups was significantly lower than in ω-3 fatty acids group ( P =0.008 ) ; meanwhile,in the ω-3 fatty acids group,the serum IL-4 levels [ (8.73 ± 1.20),(5.73 ±2.03),and (4.98 ± 1.89) pg/ml] were significantly lower (P =0.044) and serum IFN-γlevels [ (5.67 ± 0.13 ),( 6.58 ± 0.64 ),and ( 7.05 ± 0.52 ) pg/ml ] were significantly higher ( P =0.048 ) on the 72,14th,and 21st day after modeling.Conclusions ω-3 fatty acids can lower TGF-β1 protein expression in rat lung tissue and reduce the surum TGF-β1 and IL-4 levels.Compared with the middle/light fatty acids,it can more effectively upregulate the expression of IFN-γ in lung tissue and increase its serum level,and thus alleviate pulmonary fibrosis in rats.
