中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2012年
9期
1300-1301,后插1
,共3页
膀胱颈梗阻%通尿灵%再灌注损伤
膀胱頸梗阻%通尿靈%再灌註損傷
방광경경조%통뇨령%재관주손상
Urinary bladder neck obstruction%Tadenan%Reperfusion injury
目的 探讨通尿灵对兔膀胱出口部分梗阻(BOO)后逼尿肌在形态学和酶学上的保护作用.方法 28只雄性白兔随机分为四组每组7只,A组为对照组(假手术组),B、C、D组为实验组,A组行手术但不造成梗阻,B、C、D组行手术造成膀胱出口部分梗阻.术后2周起,B组给予通尿灵100 mg · kg-1·d-1口服,C组给予花生油(通尿灵赋形剂),A、D组始终不给任何药物.再饲养3周后,解剖膀胱,取膀胱逼尿肌束标本用透射电镜观察逼尿肌超微结构以及用荧光分光度计分别对MDA含量和SOD、NOS、Ca2+ -Mg2+ -ATP酶的活力进行测定.结果 D组、C组与A组相比,膀胱逼尿肌细胞中内质网明显扩张、脱颗粒,线粒体水肿明显,空泡变性和线粒体内嵴减少、消失,有的细胞内可见大量溶酶体.B组与A组比较,仅见轻度内质网扩张和线粒体水肿.各组逼尿肌中检测到的SOD[(A组:(86.568±4.657)、B组:(89.218±4.430)、C组:(3.696±3.010)、D组:(32.258±2.001) U/mgprotein)];NOS[(A组:(12.871 ±1.240)、B组:(9.274±1.137)、C组:(2.365±0.358)、D组:(3.614±0.147)U/mgprotein)]以及Ca2+-Mg2+-ATP酶[(A组:(5.231±0.329)、B组:(5.362±0.266)、C组:(2.285±0.354)、D组:(2.654±0.307) μmolPi/mgprotein)],各组逼尿肌中检测到的MDA含量[(A组:(8.369±0.170)、B组:(10.358±0.383)、C组:(36.598±3.120)、D组:(42.574±2.009)nmol/mgp)].结论 通尿灵对梗阻后逼尿肌细胞的超微结构改变具有明显保护作用.
目的 探討通尿靈對兔膀胱齣口部分梗阻(BOO)後逼尿肌在形態學和酶學上的保護作用.方法 28隻雄性白兔隨機分為四組每組7隻,A組為對照組(假手術組),B、C、D組為實驗組,A組行手術但不造成梗阻,B、C、D組行手術造成膀胱齣口部分梗阻.術後2週起,B組給予通尿靈100 mg · kg-1·d-1口服,C組給予花生油(通尿靈賦形劑),A、D組始終不給任何藥物.再飼養3週後,解剖膀胱,取膀胱逼尿肌束標本用透射電鏡觀察逼尿肌超微結構以及用熒光分光度計分彆對MDA含量和SOD、NOS、Ca2+ -Mg2+ -ATP酶的活力進行測定.結果 D組、C組與A組相比,膀胱逼尿肌細胞中內質網明顯擴張、脫顆粒,線粒體水腫明顯,空泡變性和線粒體內嵴減少、消失,有的細胞內可見大量溶酶體.B組與A組比較,僅見輕度內質網擴張和線粒體水腫.各組逼尿肌中檢測到的SOD[(A組:(86.568±4.657)、B組:(89.218±4.430)、C組:(3.696±3.010)、D組:(32.258±2.001) U/mgprotein)];NOS[(A組:(12.871 ±1.240)、B組:(9.274±1.137)、C組:(2.365±0.358)、D組:(3.614±0.147)U/mgprotein)]以及Ca2+-Mg2+-ATP酶[(A組:(5.231±0.329)、B組:(5.362±0.266)、C組:(2.285±0.354)、D組:(2.654±0.307) μmolPi/mgprotein)],各組逼尿肌中檢測到的MDA含量[(A組:(8.369±0.170)、B組:(10.358±0.383)、C組:(36.598±3.120)、D組:(42.574±2.009)nmol/mgp)].結論 通尿靈對梗阻後逼尿肌細胞的超微結構改變具有明顯保護作用.
목적 탐토통뇨령대토방광출구부분경조(BOO)후핍뇨기재형태학화매학상적보호작용.방법 28지웅성백토수궤분위사조매조7지,A조위대조조(가수술조),B、C、D조위실험조,A조행수술단불조성경조,B、C、D조행수술조성방광출구부분경조.술후2주기,B조급여통뇨령100 mg · kg-1·d-1구복,C조급여화생유(통뇨령부형제),A、D조시종불급임하약물.재사양3주후,해부방광,취방광핍뇨기속표본용투사전경관찰핍뇨기초미결구이급용형광분광도계분별대MDA함량화SOD、NOS、Ca2+ -Mg2+ -ATP매적활력진행측정.결과 D조、C조여A조상비,방광핍뇨기세포중내질망명현확장、탈과립,선립체수종명현,공포변성화선립체내척감소、소실,유적세포내가견대량용매체.B조여A조비교,부견경도내질망확장화선립체수종.각조핍뇨기중검측도적SOD[(A조:(86.568±4.657)、B조:(89.218±4.430)、C조:(3.696±3.010)、D조:(32.258±2.001) U/mgprotein)];NOS[(A조:(12.871 ±1.240)、B조:(9.274±1.137)、C조:(2.365±0.358)、D조:(3.614±0.147)U/mgprotein)]이급Ca2+-Mg2+-ATP매[(A조:(5.231±0.329)、B조:(5.362±0.266)、C조:(2.285±0.354)、D조:(2.654±0.307) μmolPi/mgprotein)],각조핍뇨기중검측도적MDA함량[(A조:(8.369±0.170)、B조:(10.358±0.383)、C조:(36.598±3.120)、D조:(42.574±2.009)nmol/mgp)].결론 통뇨령대경조후핍뇨기세포적초미결구개변구유명현보호작용.
Objective To study the protective effect of tadenan on morphologic and enzymatic changes of the detrusor muscle after bladder outlet obsttuction (BOO).Methods 28 white male rabbits were divided into four groups of 7 rabbits in each group.Control group received operation but not forming obstruction model,while the other three groups received operation forming obstruction model.Two weeks after operation,tadenan group received tadenan orall.y at 100mg · kg-1 · d -1 for three weeks;peanut oil group received vehicle only and the obstruction group did not teceive any drugs for three weeks.After three weeks,the bladder detrusor muscles of each group were collected.Morphologic changes were observed under electron microscope.The MDA concentration and the activity of SOD,NOS and Ca2 + -Mg2+ -ATP ase were measured.Results Eletron microscopy showed lots of abnormities in the detrusor muscles in obstruction group.The diltation and degranulation of rough endoplasmic reticulum were abvious.Dropsy and vecuole denaturalization were found in mitochondria.The mitochondrial crista decreased or disappeared.A plenty of lysosomes were also found in the detrusor muscle cells.The activity of SOD[A group( 86.568 ±4.657),B group(89.218 ±4.430 ),C group ( 33.696 ± 3.010 ),D group ( 32.258 ± 2.001 ) U/ng protein ],NOS [ A ( 12.87 1 ± 1.240 ),R(9.274 ± 1.137),C(2.365 ±0.358 ),D(3.614 ±0.147) U/mg protein) ] and Ca2+ -Mg2+ -ATP ase[ (A(5.231 ±0.329),R( 5.362 ± 0.266),C ( 2.285 ± 0.354 ),D ( 2.654 ± 0.307 ) μmolPi/mg protein ) ],the concentration of MDA[A(8.369 ±0.170 ),B( 10.358 ±0.383 ),C(36.598 ± 3.120),D(42.574 -± 2.009) nmol/mg protein) ].Conclusion Tadenan had the protective effect on ultrastructure of detrusor cell,and it reduce the production of free radical and avoide excessive superoxidation to prevent the process of pathologic changes of the detrusor muscles.
