CAJ | 학술논문

目的观察慢性苯中毒患者造血抑制期ICBP90的表达及慢性苯中毒患者造血恢复期ICBP90的表达,并探讨慢性苯中毒人群造血抑制与ICBP90的相关性.方法采集慢性苯中毒患者[包括造血抑制期(13例)、造血恢复期患者(11例)]和健康志愿者(10例)的骨髓,分离单个核细胞,并提取核蛋白.使用免疫印迹法(Western blot)检测核蛋白ICBP90的表达,对慢性苯中毒患者造血抑制和ICBP90表达进行相关性分析.结果慢性苯中毒造血抑制期患者骨髓单个核细胞中ICBP90表达较正常对照组明显下降,差异有统计学意义(P＜0.01).慢性苯中毒造血恢复期患者骨髓单个核细胞中ICBP90表达较正常对照组明显升高,差异有统计学意义(P＜0.05).慢性苯中毒造血恢复期患者骨髓单个核细胞中ICBP90表达较造血抑制期患者明显升高,差异亦有统计学意义(P＜0.01).慢性苯中毒所致造血抑制患者的ICBP90表达与外周血白细胞、血小板均存在相关性(r1=0.555,P=0.006; r2=0.854,P＜0.01).结论慢性苯中毒患者造血抑制期ICBP90表达下降,慢性苯中毒患者造血恢复期ICBP90表达增高.慢性苯中毒患者造血抑制与ICBP90具有相关性.
목적 관찰만성분중독환자조혈억제기ICBP90적표체급만성분중독환자조혈회복기ICBP90적표체,병탐토만성분중독인군조혈억제여ICBP90적상관성.방법 채집만성분중독환자[포괄조혈억제기(13례)、조혈회복기환자(11례)]화건강지원자(10례)적골수,분리단개핵세포,병제취핵단백.사용면역인적법(Western blot)검측핵단백ICBP90적표체,대만성분중독환자조혈억제화ICBP90표체진행상관성분석.결과 만성분중독조혈억제기환자골수단개핵세포중ICBP90표체교정상대조조명현하강,차이유통계학의의(P＜0.01).만성분중독조혈회복기환자골수단개핵세포중ICBP90표체교정상대조조명현승고,차이유통계학의의(P＜0.05).만성분중독조혈회복기환자골수단개핵세포중ICBP90표체교조혈억제기환자명현승고,차이역유통계학의의(P＜0.01).만성분중독소치조혈억제환자적ICBP90표체여외주혈백세포、혈소판균존재상관성(r1=0.555,P=0.006; r2=0.854,P＜0.01).결론 만성분중독환자조혈억제기ICBP90표체하강,만성분중독환자조혈회복기ICBP90표체증고.만성분중독환자조혈억제여ICBP90구유상관성.
Objective To observe the change of ICBP90 expression in patients with chronic benzene poisoning and explore the correlation between the expression of ICBP90 and benzene-induced hematotoxicity.Methods The bone marrow samples were from 13 chronic benzene poisoning cases with hematopoietic suppression,11 chronic benzene poisoning cases with hematopoietic regeneration and 10 controls.Western-blot was applied to detect the ICBP90 expression in bone marrow mononuclear cells (BMNCs).The correlation between ICBP90 expression and hematopoietic suppression in patients with chronic benzene poisoning was analyzed.Results The ICBP90 expression of BMNCs in 13 chronic benzene poisoning cases with hematopoietic suppression was significantly lower than that in controls (P＜0.01).The ICBP90 expression of BMNCs in 11 chronic benzene poisoning cases with hematopoietic regeneration was significantly higher than those in controls and 13 chronic benzene poisoning cases with hematopoietic suppression (P＜0.05 or P＜0.01 ),respectively.There were good correlations between the expression of ICBP90 and white blood cell and platelet counts in patients with chronic benzene poisoning (r1=0.555,P=0.006;r2=0.854,P＜0.01).Conclusion The ICBP90 expression of BMNCs in the chronic benzene poisoning cases with hematopoietic suppression decreaswed significantly,and the ICBP90 expression of BMNCs in the chronic benzene poisoning cases with hematopoietic regeneration increased significantly.There was good correlation between hematopoietic suppression and ICBP90 expression in patients with chronic benzene poisoning.