中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2010年
1期
21-24
,共4页
郑京%郑春盛%江敏%王菊霞%陈小丽%刘芳%陈雪兰%丘余良%庄玲玲%冯木兰%林善忠
鄭京%鄭春盛%江敏%王菊霞%陳小麗%劉芳%陳雪蘭%丘餘良%莊玲玲%馮木蘭%林善忠
정경%정춘성%강민%왕국하%진소려%류방%진설란%구여량%장령령%풍목란%림선충
狼疮肾炎%转化生长因子β%白细胞介素17%T淋巴细胞
狼瘡腎炎%轉化生長因子β%白細胞介素17%T淋巴細胞
랑창신염%전화생장인자β%백세포개소17%T림파세포
Lupus nephritis%Transforming growth factor beta%Interleukin-17%T lymphocytes
目的 检测活动期、静止期狼疮肾炎(LN)患者及健康人外周血白细胞介素(IL)-10、IL-6、转化生长因子(TGF)-β、IL-17、CD4~+CD25~+CD127~(lo)调节性T淋巴细胞水平,探讨TGF-β与调节性T淋巴细胞、IL-6、IL-17在LN的变化及相互关系.方法 采用三色荧光染色通过流式细胞仪(FCM)检测外周血中CD4~+CD25~+CD127~(lo)细胞水平,以双抗体夹心酶联免疫吸附(ELISA)法测定LN患者外周血中TGF-β、IL-17水平,用CBA流式蛋白分析系统检测外周血中IL-10、IL-6水平.结果 ①活动期LN患者外周血调节性T淋巴细胞低于静止期、健康组(P<0.01),静止期与健康组差异无统计学意义(P>0.05).②活动期LN患者IL-10、IL-6与静止期相比显著升高(P<0.01).③活动期LN、静止期LN、健康组TGF-β、IL-17无变化.④LN患者调节性T淋巴细胞与IL-10、IL-6、SLEDAI之间呈负相关(P<0.05);与C3、C4之间无显著相关性.⑤LN患者外周血IL-10、IL-6与SLEDAI呈正相关(P<0.01),与C3、C4均呈负相关(P<0.01).LN患者外周血SLEDAI与C3、C4均呈负相关(P<0.01).⑥LN患者调节性T淋巴细胞与TGF-β、IL-17之间无相关性.⑦TGF-β与IL-17呈正相关(P<0.01).结论 ①LN患者外周血中调节性T淋巴细胞及IL-10、IL-6、TGF-β的水平变化可作为狼疮活动性的指标.②LN患者外周血中调节性T淋巴细胞与IL-10、IL-6呈负相关.③激素有助于提高调节性T淋巴细胞,降低IL-17.④T淋巴细胞将随机体状态、局部微环境的改变以及维护机体免疫平衡的需要出现不同的极化.
目的 檢測活動期、靜止期狼瘡腎炎(LN)患者及健康人外週血白細胞介素(IL)-10、IL-6、轉化生長因子(TGF)-β、IL-17、CD4~+CD25~+CD127~(lo)調節性T淋巴細胞水平,探討TGF-β與調節性T淋巴細胞、IL-6、IL-17在LN的變化及相互關繫.方法 採用三色熒光染色通過流式細胞儀(FCM)檢測外週血中CD4~+CD25~+CD127~(lo)細胞水平,以雙抗體夾心酶聯免疫吸附(ELISA)法測定LN患者外週血中TGF-β、IL-17水平,用CBA流式蛋白分析繫統檢測外週血中IL-10、IL-6水平.結果 ①活動期LN患者外週血調節性T淋巴細胞低于靜止期、健康組(P<0.01),靜止期與健康組差異無統計學意義(P>0.05).②活動期LN患者IL-10、IL-6與靜止期相比顯著升高(P<0.01).③活動期LN、靜止期LN、健康組TGF-β、IL-17無變化.④LN患者調節性T淋巴細胞與IL-10、IL-6、SLEDAI之間呈負相關(P<0.05);與C3、C4之間無顯著相關性.⑤LN患者外週血IL-10、IL-6與SLEDAI呈正相關(P<0.01),與C3、C4均呈負相關(P<0.01).LN患者外週血SLEDAI與C3、C4均呈負相關(P<0.01).⑥LN患者調節性T淋巴細胞與TGF-β、IL-17之間無相關性.⑦TGF-β與IL-17呈正相關(P<0.01).結論 ①LN患者外週血中調節性T淋巴細胞及IL-10、IL-6、TGF-β的水平變化可作為狼瘡活動性的指標.②LN患者外週血中調節性T淋巴細胞與IL-10、IL-6呈負相關.③激素有助于提高調節性T淋巴細胞,降低IL-17.④T淋巴細胞將隨機體狀態、跼部微環境的改變以及維護機體免疫平衡的需要齣現不同的極化.
목적 검측활동기、정지기랑창신염(LN)환자급건강인외주혈백세포개소(IL)-10、IL-6、전화생장인자(TGF)-β、IL-17、CD4~+CD25~+CD127~(lo)조절성T림파세포수평,탐토TGF-β여조절성T림파세포、IL-6、IL-17재LN적변화급상호관계.방법 채용삼색형광염색통과류식세포의(FCM)검측외주혈중CD4~+CD25~+CD127~(lo)세포수평,이쌍항체협심매련면역흡부(ELISA)법측정LN환자외주혈중TGF-β、IL-17수평,용CBA류식단백분석계통검측외주혈중IL-10、IL-6수평.결과 ①활동기LN환자외주혈조절성T림파세포저우정지기、건강조(P<0.01),정지기여건강조차이무통계학의의(P>0.05).②활동기LN환자IL-10、IL-6여정지기상비현저승고(P<0.01).③활동기LN、정지기LN、건강조TGF-β、IL-17무변화.④LN환자조절성T림파세포여IL-10、IL-6、SLEDAI지간정부상관(P<0.05);여C3、C4지간무현저상관성.⑤LN환자외주혈IL-10、IL-6여SLEDAI정정상관(P<0.01),여C3、C4균정부상관(P<0.01).LN환자외주혈SLEDAI여C3、C4균정부상관(P<0.01).⑥LN환자조절성T림파세포여TGF-β、IL-17지간무상관성.⑦TGF-β여IL-17정정상관(P<0.01).결론 ①LN환자외주혈중조절성T림파세포급IL-10、IL-6、TGF-β적수평변화가작위랑창활동성적지표.②LN환자외주혈중조절성T림파세포여IL-10、IL-6정부상관.③격소유조우제고조절성T림파세포,강저IL-17.④T림파세포장수궤체상태、국부미배경적개변이급유호궤체면역평형적수요출현불동적겁화.
Objective To explore the role of CD4~+CD25~+CD127~(lo) regulatory T cells (Tregs) and inter-leukin (IL)-6, transforming growth factor beta (TGF-β), IL-17 in the pathogenesis of lupus nephritis (LN) by detecting the levels of IL-10, IL-6, TGF-β, IL-17, CD4~+CD25~+CD127~(lo) Tregs in the peripheral blood of patients with active and inactive LN. Methods Three-colour flow cytometry was used to quantitatively measure proportions of Treg cells, the levels of TGF-β, IL-17 were detected by ELISA, and the levels of IL-10, IL-6 in the peripheral blood were detected by Cytometric Bead Array System. Results ① Compared with the inactive LN and the normal controls (P<0.01), the level of CD4~+CD25~+CD127~(lo) Tregs from patients with active LN was lower(P<0.01). When compared with the normal controls, the level of CD4~+CD25~+CD127~(lo) Tregs from LN inactive patients had no significant difference (P>0.05). ② Compared with patients with inactive LN, the levels of IL-10, IL-6 was higher (P<0.01) in patients with active LN. ③ Compared with the patients with inactive LN and the normal controls, the levels of TGF-β, IL-17 was not significantly different (P>0.05). ④ The level of CD4~+CD25~+CD127~(lo) T cell was correlated negatively with the levels of IL-10, IL-6 and SLEDAI (P<0.05), and was not correlated with C3 and C4. ⑤ SLEDAI was correlated positively with the levels of IL-10 and IL-6 (P<0.01). SLEDAI and the level of IL-10 were correlated negatively with C3 and C4 (P<0.01 for both). ⑥ The level of CD4~+CD25~+CD127~(lo) Tregs from LN was not correlated with TGF-β and IL-17. ⑦ TGF-β was correlated positively with the level of IL-17. Conclusion ① The level changes of Tregs and IL-10, IL-6, TGF-β in the peripheral blood of LN can be used as the indicators for the activity status of lupus nephritis. ② Tregs and IL-10, IL-6 in the peripheral blood of LN patients is negatively correlated. ③ The glucocorticoid hormone is helpful to elevate the level of Tregs but decrease IL-17. T cell level can vary in different body status, different microenvironmental and immune status.
