中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
7期
588-591
,共4页
白念珠菌%生物膜%滞留菌%抗真菌%秀丽隐杆线虫
白唸珠菌%生物膜%滯留菌%抗真菌%秀麗隱桿線蟲
백념주균%생물막%체류균%항진균%수려은간선충
Candida albicans%Biofilm%Persisters%Antifungal%Caenorhabditis elegans
目的 念珠菌生物膜能够产生一定比例的可以耐受高浓度药物冲击的滞留菌(persisters).以秀丽隐杆线虫作为模式生物感染产生不同比例滞留菌的白念珠菌临床株并用两性霉素B处理,观察比较各组存活率,阐明滞留菌对抗真菌治疗效果的影响.方法 将同步化的成熟线虫与不同的白念珠菌临床株共培养2 h后,洗脱转移至96孔板中,加入梯度稀释的两性霉素B,并设不加药孔对照.培养5 d后计算各孔线虫存活率.结果 相同药物浓度下,感染产生高比例滞留菌的临床株组较同组不加药的线虫存活率的提高量明显低于感染低比例滞留菌组的提高量,差异有统计学意义(P＜0.01).结论 秀丽隐杆线虫是研究滞留菌和抗真菌药物药效学的可用模型;滞留菌的耐药性可能是抗真菌治疗失败和复发性感染的重要原因.
目的 唸珠菌生物膜能夠產生一定比例的可以耐受高濃度藥物遲擊的滯留菌(persisters).以秀麗隱桿線蟲作為模式生物感染產生不同比例滯留菌的白唸珠菌臨床株併用兩性黴素B處理,觀察比較各組存活率,闡明滯留菌對抗真菌治療效果的影響.方法 將同步化的成熟線蟲與不同的白唸珠菌臨床株共培養2 h後,洗脫轉移至96孔闆中,加入梯度稀釋的兩性黴素B,併設不加藥孔對照.培養5 d後計算各孔線蟲存活率.結果 相同藥物濃度下,感染產生高比例滯留菌的臨床株組較同組不加藥的線蟲存活率的提高量明顯低于感染低比例滯留菌組的提高量,差異有統計學意義(P＜0.01).結論 秀麗隱桿線蟲是研究滯留菌和抗真菌藥物藥效學的可用模型;滯留菌的耐藥性可能是抗真菌治療失敗和複髮性感染的重要原因.
목적 념주균생물막능구산생일정비례적가이내수고농도약물충격적체류균(persisters).이수려은간선충작위모식생물감염산생불동비례체류균적백념주균림상주병용량성매소B처리,관찰비교각조존활솔,천명체류균대항진균치료효과적영향.방법 장동보화적성숙선충여불동적백념주균림상주공배양2 h후,세탈전이지96공판중,가입제도희석적량성매소B,병설불가약공대조.배양5 d후계산각공선충존활솔.결과 상동약물농도하,감염산생고비례체류균적림상주조교동조불가약적선충존활솔적제고량명현저우감염저비례체류균조적제고량,차이유통계학의의(P＜0.01).결론 수려은간선충시연구체류균화항진균약물약효학적가용모형;체류균적내약성가능시항진균치료실패화복발성감염적중요원인.
Objective To illuminate the effect of persisters on antifungal therapy by infecting Caenorhabditis elegans as a live-animal model with Candida albicans isolates of different persister levels, treating them with amphotericin B and comparing the survival rate. Methods Glp-4 (bn2ts); sek-1 (km4) worms were synchronized and grown to sterile to L4-stage, put on different Candida albicans strains lawns separately for 2 h. Dispensed 15-20 worms per well of the 96-well plate, and added serial dilutions of amphotericin B for each strain group. Wells filled without any amphotericin B were used as negative controls intra-group. Incubated the plate at 25C for 5 days, counted the survival rate of each well. Results Compared with negative controls, survival rate of drug wells in each group increased. In the same drug concentration, the increase for high-persister group was significantly lower than that for low-persister group (P＜0.01). Conclusion Caenorhabditis elegans provides a model for the study of persisters and antifungal pharmacodynamics.The drug tolerance of persisters may be a critical component responsible for antifungal drug failure and relapsing infections.
