中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2012年
8期
639-642
,共4页
硫化氢%再灌注损伤%肾功能衰竭,急性
硫化氫%再灌註損傷%腎功能衰竭,急性
류화경%재관주손상%신공능쇠갈,급성
Hydrogen sulfide%Reperfusion injury%Kidney failure,acute
目的 观察不同剂量外源性硫化氢(H2S)供体硫氢化钠对大鼠肾脏缺血再灌注损伤( IRI)的保护作用.方法 健康雄性Wistar大鼠28只随机分为4组,即假手术组( Sham)、肾缺血再灌注(IR)组、硫氢化钠(NaHS)高剂量组、硫氢化钠低剂量组.大鼠右肾切除后,以NaHS作为硫化氢的供体,NaHS高、低剂量组分别经左肾动脉插管,按照1.5 μmol/min、300 nmol/min的剂量连续15 min给药,假手术组及IR组给予同体积生理盐水.停药5 min 后,NaHS组和IR组用无损伤微动脉夹夹闭左侧肾蒂45 min后解除阻断,建立大鼠急性IRI模型,假手术组不夹闭左肾动脉,其他操作同模型组.于肾脏恢复血流24h时留取血和肾组织标本,检测血清尿素氮(BUN)、血肌酐(Scr)；半定量分析肾脏病理损伤；检测肾组织H2S生成率；采用实时定量PCR法检测胱硫醚-β-合成酶(CBS)、胱硫醚-γ-裂解酶(CSE )mRNA表达.结果 与假手术组相比,IR组H2S生成率显著降低(P＜0.01);CBS、CSE mRNA表达显著下降(P＜0.01 );Scr、BUN显著升高(P＜0.01)；肾脏病理表现为急性肾小管坏死,且最严重.与IR组相比,NaHS预处理组H2S生成率升高(P＜0.05);CBS、CSE mRNA表达升高(P＜0.01 );Scr、BUN降低(P＜0.01)；病理损伤明显减轻.NaHS两个剂量组之间差异无统计学意义.结论 外源性H2S对大鼠IRI具有保护作用.
目的 觀察不同劑量外源性硫化氫(H2S)供體硫氫化鈉對大鼠腎髒缺血再灌註損傷( IRI)的保護作用.方法 健康雄性Wistar大鼠28隻隨機分為4組,即假手術組( Sham)、腎缺血再灌註(IR)組、硫氫化鈉(NaHS)高劑量組、硫氫化鈉低劑量組.大鼠右腎切除後,以NaHS作為硫化氫的供體,NaHS高、低劑量組分彆經左腎動脈插管,按照1.5 μmol/min、300 nmol/min的劑量連續15 min給藥,假手術組及IR組給予同體積生理鹽水.停藥5 min 後,NaHS組和IR組用無損傷微動脈夾夾閉左側腎蒂45 min後解除阻斷,建立大鼠急性IRI模型,假手術組不夾閉左腎動脈,其他操作同模型組.于腎髒恢複血流24h時留取血和腎組織標本,檢測血清尿素氮(BUN)、血肌酐(Scr)；半定量分析腎髒病理損傷；檢測腎組織H2S生成率；採用實時定量PCR法檢測胱硫醚-β-閤成酶(CBS)、胱硫醚-γ-裂解酶(CSE )mRNA錶達.結果 與假手術組相比,IR組H2S生成率顯著降低(P＜0.01);CBS、CSE mRNA錶達顯著下降(P＜0.01 );Scr、BUN顯著升高(P＜0.01)；腎髒病理錶現為急性腎小管壞死,且最嚴重.與IR組相比,NaHS預處理組H2S生成率升高(P＜0.05);CBS、CSE mRNA錶達升高(P＜0.01 );Scr、BUN降低(P＜0.01)；病理損傷明顯減輕.NaHS兩箇劑量組之間差異無統計學意義.結論 外源性H2S對大鼠IRI具有保護作用.
목적 관찰불동제량외원성류화경(H2S)공체류경화납대대서신장결혈재관주손상( IRI)적보호작용.방법 건강웅성Wistar대서28지수궤분위4조,즉가수술조( Sham)、신결혈재관주(IR)조、류경화납(NaHS)고제량조、류경화납저제량조.대서우신절제후,이NaHS작위류화경적공체,NaHS고、저제량조분별경좌신동맥삽관,안조1.5 μmol/min、300 nmol/min적제량련속15 min급약,가수술조급IR조급여동체적생리염수.정약5 min 후,NaHS조화IR조용무손상미동맥협협폐좌측신체45 min후해제조단,건립대서급성IRI모형,가수술조불협폐좌신동맥,기타조작동모형조.우신장회복혈류24h시류취혈화신조직표본,검측혈청뇨소담(BUN)、혈기항(Scr)；반정량분석신장병리손상；검측신조직H2S생성솔；채용실시정량PCR법검측광류미-β-합성매(CBS)、광류미-γ-렬해매(CSE )mRNA표체.결과 여가수술조상비,IR조H2S생성솔현저강저(P＜0.01);CBS、CSE mRNA표체현저하강(P＜0.01 );Scr、BUN현저승고(P＜0.01)；신장병리표현위급성신소관배사,차최엄중.여IR조상비,NaHS예처리조H2S생성솔승고(P＜0.05);CBS、CSE mRNA표체승고(P＜0.01 );Scr、BUN강저(P＜0.01)；병리손상명현감경.NaHS량개제량조지간차이무통계학의의.결론 외원성H2S대대서IRI구유보호작용.
Objective To observe the protective effect of different doses sodium hydrosulfide (NaHS) as a donor of hydrogen sulfide on renal ischemia reperfusion injury (IRI).Methods Twenty-eight healthy male Wistar rats were randomly divided into 4 groups,namely sham operation (Sham) group (n =7),renal ischemia reperfusion (IR) group (n =7),sodium hydrosulfide (NaHS) high dose group (n=7),sodium hydrosulfide low dose group (n=7).After excision of the right kidney,two dose NaHS group (300 nmol/min,1.5 μmol/min) received 15-minute continuous administration via left renal artery.Sham group and IR group received same volume of saline.Five minutes after stopping drug,the left renal pedicle in NaHS group and IR group was clipped with no damage arteriole occlusion.After 45 minutes blockade,a model of acute renal ischemia reperfusion injury was established.Sham group underwent the same procedure as model groups without clamping the left renal artery.Specimens of renal tissue and blood were harvested at 24-hour after blood flow restore in the kidney.BUN and Scr were measured.Kidney pathological damage was semi-quantitatively analyzed.Production of H2S in renal tissue was detected.Expression of cystathionine γ lyase (CSE) and cystathionine β synthase (CBS) mRNA in kidney tissue was examined by real-time PCR.Results Compared with sham group,BUN and Scr increased significantly (P＜0.01),kidney tissue H2S production decreased significantly (P＜0.01),mRNA expression of CSE and CBS down-regulated significantly (P＜0.01) in IR group.The kidney pathology of sham group was normal,while acute tubular necrosis was found in IR group.Compared with IR group,BUN and Scr decreased significantly (P＜0.01),kidney tissue H2S production increased significantly (P＜0.05),mRNA expression of CSE and CBS up-regulated significantly (P＜0.01) in NaHS groups.Pathological damage of acute tubular necrosis was significantly improved in NaHS pretreatment group.There was no significant difference between two NaHS doses groups.Conclusion Hydrogen sulfide has a protective effect on renal IRI.
